รศ.ดร.นพ. ชัชวาลย์ ศรีสวัสดิ์

  • พบ. (เกียรตินิยมอันดับ 1) คณะแพทยศาสตร์ศิริราชพยาบาล มหาวิทยาลัยมหิดล
  • Ph.D. (Biological Chemistry), University of Michigan (Ann Arbor)

Office : 1016 อาคารศรีสวรินทิรา ชั้น 10 ภาควิชาชีวเคมี คณะแพทยศาสตร์ศิริราชพยาบาล โรงพยาบาลศิริราช, เขตบางกอกน้อย, กรุงเทพฯ 10700

Laboratory : 916 อาคารศรีสวรินทิรา ชั้น 9

Phone : (02) 419-9144

E-Mail : chatchawan.sri@mahidol.ac.th

งานวิจัย

Aptamer เป็น DNA หรือ RNA ที่มีคุณสมบัติพิเศษคือสามารถจับกับโมเลกุล ของสารต่างๆได้อย่างจำเพาะ ถึงแม้ว่าโมเลกุลนั้นจะไม่ใช่สารที่จับอยู่กับ DNA หรือ RNA ในธรรมชาติ ในปัจจุบันได้มีการผลิต aptamer ที่มีความจำเพาะกับโมเลกุลชนิดต่างๆ หลายชนิดตั้งแต่สารโมเลกุลเล็ก เช่น ยา หรือ กรดอะมิโน ไปจนถึงสาร macromolecule เช่น คาร์โบไฮเดรต หรือ โปรตีน ซึ่ง aptamer ที่ผลิตได้ดังกล่าวสามารถนำไปใช้ได้ในลักษณะเดียวกับ monoclonal antibody เช่น ใช้ในด้านการวิจัย, การวินิจฉัยโรค และ การรรักษา โดยในปัจจุบันได้มีการผลิต therapeutic aptamer หลายชนิด (เช่น aptamer ต่อ vascular endothelial growth factor) ที่แสดงให้เห็นว่ามีบทบาทในการรักษาที่ได้ผล และกำลังถูกทดสอบในขั้น clinical trial เพื่อพัฒนาใช้เป็น therapeutic agent ต่อไป
การผลิต aptamer เป็นเทคโนโลยีใหม่ที่ได้รับการพัฒนา ตลอดจนนำไปใช้ ในด้านต่างๆอย่างแพร่หลาย ในช่วงเวลาไม่กี่ปีที่ผ่านมา การผลิต aptamer สามารถทำได้โดยการคัดเลือก DNA หรือ RNA ที่มีคุณสมบัติในการจับกับ โมเลกุลที่ต้องการจาก library ของ DNA หรือ RNA ที่มีลำดับเบส ที่มีความแตกต่างและซับซ้อนอย่างมากด้วยเทคนิค SELEX หรือ Systematic Evolution of Ligands by EXponential enrichment (ดังรูป) ทำให้ได้ DNA หรือ RNA ligand (aptamer) ที่มีคุณสมบัติ ที่ต้องการคล้ายกับ monoclonal antibody แต่ขบวนการผลิตทุกขั้นตอนทำให้หลอดทดลอง ทำให้ไม่ต้อง ใช้สัตว์ทดลอง หรือใช้เวลาในการผลิตนานเหมือนการผลิต monoclonal antibody นอกจากนี้ ขบวนการผลิตยังมีราคาที่ถูกกว่าและผลิตได้ง่ายกว่า ทำให้ aptamer กำลังเข้ามามีบทบาทสำคัญ และเป็นเทคโนโลยีที่นักวิทยาศาสตร์ให้ความสนใจ ในการที่จะพัฒนาเพื่อใช้ร่วมกับหรือทดแทน monoclonal antibody ในอนาคต

การผลิต aptamer เป็นเทคโนโลยีใหม่ที่ได้รับการพัฒนาตลอดจนนำไปใช้ในด้านต่างๆอย่างแพร่หลายในช่วงเวลาไม่กี่ปีที่ผ่านมา การผลิต aptamer สามารถทำได้โดยการคัดเลือก DNA หรือ RNA ที่มีคุณสมบัติในการจับกับโมเลกุลที่ต้องการจาก library ของ DNA หรือ RNA ที่มีลำดับเบสที่มีความแตกต่างและซับซ้อนอย่างมาก ด้วยเทคนิค SELEX หรือ Systematic Evolution of Ligands by EXponential enrichment (ดังรูป) ทำให้ได้ DNA หรือ RNA ligand (aptamer) ที่มีคุณสมบัติที่ต้องการคล้ายกับ monoclonal antibody แต่ขบวนการผลิตทุกขั้นตอน ทำในหลอดทดลอง ทำให้ไม่ต้องใช้สัตว์ทดลอง หรือใช้เวลาในการผลิตนานเหมือนการผลิต monoclonal antibody นอกจากนี้ขบวนการผลิตยังมีราคาที่ถูกกว่า และผลิตได้ง่ายกว่า ทำให้ aptamer กำลังเข้ามามีบทบาทสำคัญ และเป็นเทคโนโลยี ที่นักวิทยาศาสตร์ให้ความสนใจในการที่จะพัฒนา เพื่อใช้ร่วมกับหรือทดแทน monoclonal antibody ในอนาคต
โครงการวิจัยเกี่ยวกับ aptamer ที่กำลังดำเนินอยู่ในขณะนี้ มีวัตถุประสงค์เพื่อพัฒนา RNA aptamer ที่มีความจำเพาะกับ envelope protein ของเชื้อไวรัสไข้เลือดออก และสามารถมีฤทธิ์ยับยั้งการติดเชื้อไวรัสต่อเซลล์ได้ ซึ่ง aptamer ดังกล่าวอาจจะมีประโยชน์ในการวินิจฉัย หรือการรักษาโรคดังกล่าวในอนาคต นอกจากนี้ ยังมี

โครงการพัฒนา aptamer ที่มีความจำเพาะกับ hemoglobin ชนิดต่างๆ ซึ่ง RNA aptamer ดังกล่าวอาจมีบทบาทสำคัญในการวินิจฉัยภาวะ hemoglobinopathy หรือ thalassemia ซึ่งเป็นโรคทางโลหิตวิทยาที่พบได้บ่อย สำหรับทิศทางของโครงการวิจัยในอนาคต จะมีการพัฒนา aptamer สำหรับ target molecule อื่นๆเพื่อใช้ในแง่ diagnostic หรือ therapeutic สำหรับโรคที่พบบ่อยและมีความสำคัญในประเทศต่อไป

นอกจากนี้ ในห้องปฏิบัติการยังมีโครงการวิจัยเกี่ยวกับการยับยั้งการสังเคราะห์คอลลาเจนในเซลล์ keloid fibroblastโดยใช้ curcumin และ RNA interference ต่อ collagen genes (COL1A1 และ COL1A2) ซึ่งผลการทดลองอาจจะมีประโยชน์ในแง่ของการรักษาแผลนูน keloid ต่อไปในอนาคต

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13. Atchaneeyasakul, L. O., Appukuttan, B., Pingsuthiwong, S., Yenchitsomanus, P. T., Trinavarat, A., Srisawat, C., & Study Group (2006). A novel H572R mutation in the transforming growth factor-beta-induced gene in a Thai family with lattice corneal dystrophy type I. Japanese journal of ophthalmology, 50(5), 403–408. https://doi.org/10.1007/s10384-006-0357-6
14. Peerapittayamongkol, C., Srisawat, C., Junnu, S., Kanyok, S., Soiampornkul, R., Poonsawas, I., et al. (2007)Porphyrias diagnosed types by HPLC analysis: 10 years Siriraj experience. Med J 2007;59:356-60.
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17. Limjindaporn, T., Wongwiwat, W., Noisakran, S., Srisawat, C., Netsawang, J., Puttikhunt, C., Kasinrerk, W., Avirutnan, P., Thiemmeca, S., Sriburi, R., Sittisombut, N., Malasit, P., & Yenchitsomanus, P. T. (2009). Interaction of dengue virus envelope protein with endoplasmic reticulum-resident chaperones facilitates dengue virus production. Biochemical and biophysical research communications, 379(2), 196–200. https://doi.org/10.1016/j.bbrc.2008.12.070
18. Kirawanich, P., Pausawasdi, N., Srisawat, C., Yakura, SJ., Islam, NE. (2009). An FDTD Interaction Scheme of a High-Intensity Nanosecond-Pulsed Electric-Field System for In Vitro Cell Apoptosis Applications. IEEE Transactions on Plasma Science 2010;38 (10 PART 1), art. no. 5404942, pp. 2574-82.
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22. Moongkarndi, P., Srisawat, C., Saetun, P., Jantaravinid, J., Peerapittayamongkol, C., Soi-ampornkul, R., Junnu, S., Sinchaikul, S., Chen, S. T., Charoensilp, P., Thongboonkerd, V., & Neungton, N. (2010). Protective effect of mangosteen extract against beta-amyloid-induced cytotoxicity, oxidative stress and altered proteome in SK-N-SH cells. Journal of proteome research, 9(5), 2076–2086. https://doi.org/10.1021/pr100049v
23. Jungtrakoon, P., Plengvidhya, N., Tangjittipokin, W., Chimnaronk, S., Salaemae, W., Chongjaroen, N., Chanprasert, K., Sujjitjoon, J., Srisawat, C., & Yenchitsomanus, P. T. (2011). Novel adiponectin variants identified in type 2 diabetic patients reveal multimerization and secretion defects. PloS one, 6(10), e26792. https://doi.org/10.1371/journal.pone.0026792
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25. Morchang, A., Yasamut, U., Netsawang, J., Noisakran, S., Wongwiwat, W., Songprakhon, P., Srisawat, C., Puttikhunt, C., Kasinrerk, W., Malasit, P., Yenchitsomanus, P. T., & Limjindaporn, T. (2011). Cell death gene expression profile: Role of RIPK2 in dengue virus-mediated apoptosis. Virus Research, 156(1–2), 25–34. https://doi.org/10.1016/j.virusres.2010.12.012
26. Ratchatawipasanan, C., Sirivattanauksorn, V., Srisawat, C., Chuthapisith, S. (2012). Roles of circulating tumour cells in breast cancer. J Assoc Gen Surg Thailand 2012;8(17):44-48
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31. Rungtip Soi-ampornkul, Chatchawan Srisawat, Wipawan Thangnipon, Surin Kanyok, Sarawut Junnu, et al. (2013). Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells. International Journal of Nutrition and Food Sciences, 2(4), 167-173. https://doi.org/10.11648/j.ijnfs.20130204.12
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34. Sreekanth, G. P., Chuncharunee, A., Sirimontaporn, A., Panaampon, J., Srisawat, C., Morchang, A., Malakar, S., Thuwajit, P., Kooptiwut, S., Suttitheptumrong, A., Songprakhon, P., Noisakran, S., Yenchitsomanus, P. T., & Limjindaporn, T. (2014). Role of ERK1/2 signaling in dengue virus-induced liver injury. Virus research, 188, 15–26. https://doi.org/10.1016/j.virusres.2014.03.025
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38. Srisawat, C., Nakponetong, K., Benjasupattananun, P., Suratannon, C., Wachirutmanggur, L., Boonchoo, S., Pankaew, D., Laocharoenkiat, A., Pacharn, P., Jirapongsananuruk, O., Visitsunthorn, N., & Vichyanond, P. (2016). A preliminary study of intranasal epinephrine administration as a potential route for anaphylaxis treatment. Asian Pacific journal of allergy and immunology, 34(1), 38–43.
39. Kooptiwut, S., Wanchai, K., Semprasert, N., Srisawat, C., & Yenchitsomanus, P. T. (2017). Estrogen attenuates AGTR1 expression to reduce pancreatic β-cell death from high glucose. Scientific reports, 7(1), 16639. https://doi.org/10.1038/s41598-017-15237-4
40. Tummarintra, P., Limratana, P., Sujirattanawimol, K., & Srisawat, C. (2018). The Contamination of Intravenous Fluid by Felt-Tip Marking Pen Ink: A Pilot Study. Siriraj Medical Journal, 70(4), 349–354. retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/153939
41. Junnu, S., Srisawat, C. (2018). Porphyria Laboratory Diagnostics in Thailand. The International Journal of the Royal Society of Thailand.2018;10:96-108
42. Trakarnsanga, K., Wilson, M. C., Heesom, K. J., Andrienko, T. N., Srisawat, C., & Frayne, J. (2018). Secretory factors from OP9 stromal cells delay differentiation and increase the expansion potential of adult erythroid cells in vitro. Scientific reports, 8(1), 1983. https://doi.org/10.1038/s41598-018-20491-1
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44. Napatpittayatorn, P., Kritpet, T., Muangpaisan, W., Srisawat, C., & Junnu, S. (2019). Effects of neurobic exercise on cognitive function and serum brain-derived neurotrophic factor in the normal to mild cognitive impaired older people: A randomized control trial. Songklanakarin Journal of Science and Technology, 41(3), 551-558. https://doi.org/10.14456/sjst-psu.2019.74
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50. Trakarnsanga, K., Tipgomut, C., Metheetrairut, C., Wattanapanitch, M., Khuhapinant, A., Poldee, S., Kurita, R., Nakamura, Y., Srisawat, C., & Frayne, J. (2020). Generation of an immortalised erythroid cell line from haematopoietic stem cells of a haemoglobin E/β-thalassemia patient. Scientific reports, 10(1), 16798. https://doi.org/10.1038/s41598-020-73991-4
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52. Kongmalai, T., Preechasuk, L., Junnu, S., Manocheewa, S., Srisawat, C., & Sriwijitkamol, A. (2021). The Effect of Temperature on the Stability of In-Use Insulin Pens. Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association, 129(9), 683–688. https://doi.org/10.1055/a-1010-5466
53. Jantaravinid, J., Jiamton, S., Srisawat, C., Suktitipat, B., & Tirawanchai, N. (2021). Expression Pattern of Genes in Condyloma Acuminata Treated with Clinacanthus nutans Lindau Cream versus Podophyllin. Evidence-based complementary and alternative medicine : eCAM, 2021, 5579520. https://doi.org/10.1155/2021/5579520
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57. Chularojanamontri, L., Wongpraparut, C., Silpa-Archa, N., Chaiyabutr, C., Pruksaeakanan, C., Klinniyom, A., Junnu, S., & Srisawat, C. (2022). Patient-ready syringes containing 25 mg/mL methotrexate can be kept at temperature ranging from 4 °C to 37 °C for up to 12 weeks for use in psoriatic and rheumatologic conditions. The Journal of dermatological treatment, 33(2), 1023–1028. https://doi.org/10.1080/09546634.2020.1800570
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61. Mungchan, P., Glab-Ampai, K., Chruewkamlow, N., Trakarnsanga, K., Srisawat, C., Nguyen, K. T., Chaicumpa, W., & Punnakitikashem, P. (2022). Targeted Nanoparticles for the Binding of Injured Vascular Endothelium after Percutaneous Coronary Intervention. Molecules (Basel, Switzerland), 27(23), 8144. https://doi.org/10.3390/molecules27238144
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