{"id":4522,"date":"2025-12-17T10:55:26","date_gmt":"2025-12-17T03:55:26","guid":{"rendered":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/?p=4522"},"modified":"2026-03-30T15:26:31","modified_gmt":"2026-03-30T08:26:31","slug":"angiotensin-ii-promotes-erythroid-proliferation-in-a-three-stage-erythroid-culture-system","status":"publish","type":"post","link":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/en\/angiotensin-ii-promotes-erythroid-proliferation-in-a-three-stage-erythroid-culture-system\/","title":{"rendered":"Angiotensin II promotes erythroid proliferation in a three-stage erythroid culture system"},"content":{"rendered":"

Saiphon Poldee<\/strong> , Chanatip Metheetrairut<\/strong> , Wichit Suthammarak<\/strong>, Kongtana Trakarnsanga<\/strong> <\/p>\n

Abstract<\/h2>\n
\n
\n

At present, the numbers of cultured erythroid cells obtained from culture systems are not on a scale that can be used for therapeutics since the cultured erythroid cells have limited proliferation capacity. Stromal cells are believed to play important roles during erythropoiesis. Our previous study shows that factors secreted by stromal cells enhance the proliferation capacity of adult erythroid cells in the culture system. Among the identified factors, angiotensinogen is one of the most abundant proteins secreted by the stromal cells. This study aims to investigate the effect of angiotensin II, an angiotensinogen derivative, on the proliferation of erythroid cells. \u2022The receptor for angiotensin II was first checked by PCR analysis. It was expressed in erythroblasts at all stages during differentiation.\u2022To study the effect of angiotensin II, CD34+ hematopoietic stem cells were cultured in a 3-stage erythroid culture system with and without angiotensin II. The addition of angiotensin II to the culture media, from day 0 to 8, significantly increases the numbers of cultured erythroid cells, whereas no difference in enucleation is observed.<\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/38660027\/<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Saiphon Poldee , Chanatip Metheetrairut , Wichit Suthammarak, Kongtana Trakarnsanga Abstract At present, the numbers of cultured erythroid cells obtained from culture systems are not on a scale that can be used for therapeutics since the cultured erythroid cells have limited proliferation capacity. Stromal cells are believed to play important roles during erythropoiesis. 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