{"id":5373,"date":"2026-02-25T09:36:27","date_gmt":"2026-02-25T02:36:27","guid":{"rendered":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/?p=5373"},"modified":"2026-03-30T13:23:45","modified_gmt":"2026-03-30T06:23:45","slug":"extracellular-vesicles-a-comprehensive-review-of-classification-isolation-characterization-and-cargo-loading","status":"publish","type":"post","link":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/en\/extracellular-vesicles-a-comprehensive-review-of-classification-isolation-characterization-and-cargo-loading\/","title":{"rendered":"Extracellular vesicles: A comprehensive review of classification, isolation, characterization, and cargo loading"},"content":{"rendered":"

Chanatip Metheetrairut<\/strong>, Ladawan Khowawisetsut, Punnida Nonsuwan<\/strong>, Primana Punnakitikashema<\/strong>, Kovit Pattanapanyasat<\/p>\n

Abstract<\/h2>\n
\n
\n

Extracellular vesicles (EVs) are submicron membrane-bound structures released from various cell types into extracellular space. EVs are divided by biogenesis into exosomes, microvesicles and apoptotic bodies; moreover, they can also be subtyped into natural, engineered and hybrid EVs. EVs play a vital role in cell-to-cell communication, allowing cells to exchange cargos including proteins, lipids and nucleic acid materials, therefore making them valuable tools as novel biomarkers of diseases, as therapeutic agents and as drug delivery messengers. In this review, we describe several methods for isolation and characterization of EVs. Furthermore, engineered EVs as target drug delivery systems as well as recent advances in hybrid EVs and the engineering of EVs with synthetic lipid nanoparticles will also be discussed. \u00a9 2025 Science Society of Thailand under Royal Patronage. All rights reserved.<\/p>\n

https:\/\/www.scopus.com\/pages\/publications\/105009992206?origin=resultslist<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Chanatip Metheetrairut, Ladawan Khowawisetsut, Punnida Nonsuwan, Primana Punnakitikashema, Kovit Pattanapanyasat Abstract Extracellular vesicles (EVs) are submicron membrane-bound structures released from various cell types into extracellular space. EVs are divided by biogenesis into exosomes, microvesicles and apoptotic bodies; moreover, they can also be subtyped into natural, engineered and hybrid EVs. EVs play a vital role in […]\n","protected":false},"author":6,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-5373","post","type-post","status-publish","format-standard","hentry","category-research-innovation-en"],"yoast_head":"\nExtracellular vesicles: A comprehensive review of classification, isolation, characterization, and cargo loading - \u0e20\u0e32\u0e04\u0e27\u0e34\u0e0a\u0e32\u0e0a\u0e35\u0e27\u0e40\u0e04\u0e21\u0e35 \u0e04\u0e13\u0e30\u0e41\u0e1e\u0e17\u0e22\u0e28\u0e32\u0e2a\u0e15\u0e23\u0e4c\u0e28\u0e34\u0e23\u0e34\u0e23\u0e32\u0e0a\u0e1e\u0e22\u0e32\u0e1a\u0e32\u0e25 \u0e21\u0e2b\u0e32\u0e27\u0e34\u0e17\u0e22\u0e32\u0e25\u0e31\u0e22\u0e21\u0e2b\u0e34\u0e14\u0e25<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/en\/extracellular-vesicles-a-comprehensive-review-of-classification-isolation-characterization-and-cargo-loading\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Extracellular vesicles: A comprehensive review of classification, isolation, characterization, and cargo loading - \u0e20\u0e32\u0e04\u0e27\u0e34\u0e0a\u0e32\u0e0a\u0e35\u0e27\u0e40\u0e04\u0e21\u0e35 \u0e04\u0e13\u0e30\u0e41\u0e1e\u0e17\u0e22\u0e28\u0e32\u0e2a\u0e15\u0e23\u0e4c\u0e28\u0e34\u0e23\u0e34\u0e23\u0e32\u0e0a\u0e1e\u0e22\u0e32\u0e1a\u0e32\u0e25 \u0e21\u0e2b\u0e32\u0e27\u0e34\u0e17\u0e22\u0e32\u0e25\u0e31\u0e22\u0e21\u0e2b\u0e34\u0e14\u0e25\" \/>\n<meta property=\"og:description\" content=\"Chanatip Metheetrairut, Ladawan Khowawisetsut, Punnida Nonsuwan, Primana Punnakitikashema, Kovit Pattanapanyasat Abstract Extracellular vesicles (EVs) are submicron membrane-bound structures released from various cell types into extracellular space. 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