{"id":5384,"date":"2026-02-25T09:42:46","date_gmt":"2026-02-25T02:42:46","guid":{"rendered":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/?p=5384"},"modified":"2026-03-30T13:21:17","modified_gmt":"2026-03-30T06:21:17","slug":"a-proof-of-concept-study-using-a-graphene-oxide-bovine-serum-albumin-nanocomposite-based-electrochemical-aptasensor-platform-for-salmonella-typhimurium-detection-from-chicken-meat","status":"publish","type":"post","link":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/en\/a-proof-of-concept-study-using-a-graphene-oxide-bovine-serum-albumin-nanocomposite-based-electrochemical-aptasensor-platform-for-salmonella-typhimurium-detection-from-chicken-meat\/","title":{"rendered":"A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat"},"content":{"rendered":"<p>Settasart Sonna, <strong>Chatchawan Srisawat<\/strong>, Sakda Jampasa, Orawon Chailapakul, David J Hampson , Nuvee Prapasarakul<\/p>\n<h2 class=\"title\">Abstract<\/h2>\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\n<div class=\"title\">\n<p>Salmonella Typhimurium (ST) is an important food-borne pathogen, particularly in chicken meat, making its rapid detection essential for food safety. Conventional detection methods are slow and equipment-intensive, creating the need for sensitive and portable alternatives. We developed a rapid, ultrasensitive, and portable electrochemical aptasensor platform for detecting ST in samples extracted from chicken meat. The sensor employed graphene oxide-bovine serum albumin (GO-BSA) nanocomposite to enhance electrode biocompatibility, stability, and aptamer immobilization. A specific NH2-modified DNA aptamer targeting ST allowed direct binding without prior extraction. Fabrication steps included GO-BSA drop-casting, aptamer immobilization, and BSA blocking before ST binding. The sensor operates on a signal-off mechanism, measured via differential pulse voltammetry (DPV), where the aptamer-ST complex formation reduces the redox signal of [Fe(CN)6]\u00b3-\/4- due to insulation and electrostatic repulsion. The aptasensor demonstrated excellent sensitivity (limit of detection 3 CFU\/mL) and high specificity. Performance validation using spiked samples extracted from chicken meat confirmed the effective detection of ST in a complex matrix, aligning with the results obtained through culture-based methods. The sensor also maintained good stability for up to 28 days at 4 \u00b0C. These results showed that aptasensors can be a rapid, cost-effective, and field-deployable tool for monitoring foodborne pathogens.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/41287526\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/41287526\/<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Settasart Sonna, Chatchawan Srisawat, Sakda Jampasa, Orawon Chailapakul, David J Hampson , Nuvee Prapasarakul Abstract Salmonella Typhimurium (ST) is an important food-borne pathogen, particularly in chicken meat, making its rapid detection essential for food safety. Conventional detection methods are slow and equipment-intensive, creating the need for sensitive and portable alternatives. We developed a rapid, ultrasensitive, [&#8230;]\n","protected":false},"author":6,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-5384","post","type-post","status-publish","format-standard","hentry","category-research-innovation-en"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat - \u0e20\u0e32\u0e04\u0e27\u0e34\u0e0a\u0e32\u0e0a\u0e35\u0e27\u0e40\u0e04\u0e21\u0e35 \u0e04\u0e13\u0e30\u0e41\u0e1e\u0e17\u0e22\u0e28\u0e32\u0e2a\u0e15\u0e23\u0e4c\u0e28\u0e34\u0e23\u0e34\u0e23\u0e32\u0e0a\u0e1e\u0e22\u0e32\u0e1a\u0e32\u0e25 \u0e21\u0e2b\u0e32\u0e27\u0e34\u0e17\u0e22\u0e32\u0e25\u0e31\u0e22\u0e21\u0e2b\u0e34\u0e14\u0e25<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/en\/a-proof-of-concept-study-using-a-graphene-oxide-bovine-serum-albumin-nanocomposite-based-electrochemical-aptasensor-platform-for-salmonella-typhimurium-detection-from-chicken-meat\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat - \u0e20\u0e32\u0e04\u0e27\u0e34\u0e0a\u0e32\u0e0a\u0e35\u0e27\u0e40\u0e04\u0e21\u0e35 \u0e04\u0e13\u0e30\u0e41\u0e1e\u0e17\u0e22\u0e28\u0e32\u0e2a\u0e15\u0e23\u0e4c\u0e28\u0e34\u0e23\u0e34\u0e23\u0e32\u0e0a\u0e1e\u0e22\u0e32\u0e1a\u0e32\u0e25 \u0e21\u0e2b\u0e32\u0e27\u0e34\u0e17\u0e22\u0e32\u0e25\u0e31\u0e22\u0e21\u0e2b\u0e34\u0e14\u0e25\" \/>\n<meta property=\"og:description\" content=\"Settasart Sonna, Chatchawan Srisawat, Sakda Jampasa, Orawon Chailapakul, David J Hampson , Nuvee Prapasarakul Abstract Salmonella Typhimurium (ST) is an important food-borne pathogen, particularly in chicken meat, making its rapid detection essential for food safety. Conventional detection methods are slow and equipment-intensive, creating the need for sensitive and portable alternatives. We developed a rapid, ultrasensitive, [...]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/en\/a-proof-of-concept-study-using-a-graphene-oxide-bovine-serum-albumin-nanocomposite-based-electrochemical-aptasensor-platform-for-salmonella-typhimurium-detection-from-chicken-meat\/\" \/>\n<meta property=\"og:site_name\" content=\"\u0e20\u0e32\u0e04\u0e27\u0e34\u0e0a\u0e32\u0e0a\u0e35\u0e27\u0e40\u0e04\u0e21\u0e35 \u0e04\u0e13\u0e30\u0e41\u0e1e\u0e17\u0e22\u0e28\u0e32\u0e2a\u0e15\u0e23\u0e4c\u0e28\u0e34\u0e23\u0e34\u0e23\u0e32\u0e0a\u0e1e\u0e22\u0e32\u0e1a\u0e32\u0e25 \u0e21\u0e2b\u0e32\u0e27\u0e34\u0e17\u0e22\u0e32\u0e25\u0e31\u0e22\u0e21\u0e2b\u0e34\u0e14\u0e25\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/SIBBprogram\/\" \/>\n<meta property=\"article:published_time\" content=\"2026-02-25T02:42:46+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-03-30T06:21:17+00:00\" \/>\n<meta name=\"author\" content=\"Poorintra Sakuna\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Poorintra Sakuna\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www2.si.mahidol.ac.th\\\/department\\\/biochemistry\\\/en\\\/a-proof-of-concept-study-using-a-graphene-oxide-bovine-serum-albumin-nanocomposite-based-electrochemical-aptasensor-platform-for-salmonella-typhimurium-detection-from-chicken-meat\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www2.si.mahidol.ac.th\\\/department\\\/biochemistry\\\/en\\\/a-proof-of-concept-study-using-a-graphene-oxide-bovine-serum-albumin-nanocomposite-based-electrochemical-aptasensor-platform-for-salmonella-typhimurium-detection-from-chicken-meat\\\/\"},\"author\":{\"name\":\"Poorintra Sakuna\",\"@id\":\"https:\\\/\\\/www2.si.mahidol.ac.th\\\/department\\\/biochemistry\\\/en\\\/#\\\/schema\\\/person\\\/75170c4a414d080cfd0b7428b2e0d11a\"},\"headline\":\"A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat\",\"datePublished\":\"2026-02-25T02:42:46+00:00\",\"dateModified\":\"2026-03-30T06:21:17+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www2.si.mahidol.ac.th\\\/department\\\/biochemistry\\\/en\\\/a-proof-of-concept-study-using-a-graphene-oxide-bovine-serum-albumin-nanocomposite-based-electrochemical-aptasensor-platform-for-salmonella-typhimurium-detection-from-chicken-meat\\\/\"},\"wordCount\":240,\"publisher\":{\"@id\":\"https:\\\/\\\/www2.si.mahidol.ac.th\\\/department\\\/biochemistry\\\/en\\\/#organization\"},\"articleSection\":[\"Research Innovation\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www2.si.mahidol.ac.th\\\/department\\\/biochemistry\\\/en\\\/a-proof-of-concept-study-using-a-graphene-oxide-bovine-serum-albumin-nanocomposite-based-electrochemical-aptasensor-platform-for-salmonella-typhimurium-detection-from-chicken-meat\\\/\",\"url\":\"https:\\\/\\\/www2.si.mahidol.ac.th\\\/department\\\/biochemistry\\\/en\\\/a-proof-of-concept-study-using-a-graphene-oxide-bovine-serum-albumin-nanocomposite-based-electrochemical-aptasensor-platform-for-salmonella-typhimurium-detection-from-chicken-meat\\\/\",\"name\":\"A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat - 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Conventional detection methods are slow and equipment-intensive, creating the need for sensitive and portable alternatives. 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