{"id":5414,"date":"2026-02-25T09:54:05","date_gmt":"2026-02-25T02:54:05","guid":{"rendered":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/?p=5414"},"modified":"2026-03-30T11:22:33","modified_gmt":"2026-03-30T04:22:33","slug":"saccharification-of-carrot-pomace-and-use-as-nutrient-source-for-the-production-of-%ea%9e%b5-cryptoxanthin-by-pantoea-anthophila-fl1_is5","status":"publish","type":"post","link":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/en\/saccharification-of-carrot-pomace-and-use-as-nutrient-source-for-the-production-of-%ea%9e%b5-cryptoxanthin-by-pantoea-anthophila-fl1_is5\/","title":{"rendered":"Saccharification of carrot pomace and use as nutrient source for the production of \ua7b5-cryptoxanthin by Pantoea anthophila FL1_IS5"},"content":{"rendered":"
Sopida Korkerd, Savitri Vatanyoopaisarn, Wonnop Visessanguan<\/strong>, Benjawan Thumthanarak, Caroline L. Perez, Vilai Rungsardthong, Solange I. Mussatto<\/p>\n Carrot pomace represents a rich source of nutritional components such as polysaccharides, dietary fiber, phenolic compounds, and \ua7b5-carotene, which can be utilized as alternative sources to produce value-added products. In this research, carrot pomace was hydrolyzed to release monosaccharides and utilized to produce \ua7b5-carotene and \ua7b5-cryptoxanthin. The hydrolysate obtained from 10% solid loading (w\/v) and a hydrolysis time of 48 h contained glucose, xylose, and \ua7b5-carotene at 39.02 g\/L, 26.63 g\/L, and 26.65 mg\/L, respectively. This hydrolysate was supplemented as a co-substrate in the cultivation to increase cell biomass and carotenoids production by the bacterial strain Pantoea anthophila FL1_IS5. Optimization of the medium composition and the initial pH revealed that the cell biomass, \ua7b5-carotene, and \ua7b5-cryptoxanthin reached concentrations of 5.74 g\/L, 73.86 mg\/L, and 31.87 mg\/L, respectively, with 22.93% (v\/v) carrot pomace hydrolysate and 0.51% (w\/v) yeast extract, with the initial pH 7.44. This finding indicates that glucose, xylose, and \ua7b5-carotene from carrot pomace hydrolysate can be used to produce \ua7b5-cryptoxanthin by P. anthophilia, offering new opportunities for the valorization of carrot pomace.<\/p>\nAbstract<\/h2>\n