คณาจารย์ ภาควิชาชีวเคมีรศ. ดร. นภัทรธรา (เนตรนภิส) ธีระวัลย์ชัย
- Ph.D. (Parasite Biochemistry), Imperial College, University of London, UK.
Office : 1020 อาคารศรีสวรินทิรา ชั้น 10 ภาควิชาชีวเคมี คณะแพทยศาสตร์ศิริราชพยาบาล, โรงพยาบาลศิริราช, เขตบางกอกน้อย, กรุงเทพฯ 10700
Laboratory : 923 อาคารศรีสวรินทิรา ชั้น 9
Phone : (02) 419-9133
E-Mail : nednapis.tir@mahidol.ac.th
Stem cell เป็นเซลล์ที่มีคุณสมบัติในการเจริญเติบโต และมีการ differentiate ไปเป็นเซลล์อื่นๆได้เมื่อมีสภาวะที่เหมาะสม จำแนกออกได้เป็น adult และ embryonic stem cells เนื่องจาก adult stem cell เป็นเซลล์ที่มีจำนวนน้อย และต้องแยกออกมาจากร่างกายของสิ่งมีชีวิต ปัจจุบันนี้จึงนิยมสร้าง embryonic stem cell แทน เนื่องจากการสร้าง embryonic stem cell ของคน ยังมีปัญหาเรื่องจริยธรรม การสร้าง embryonic stem cell โดยใช้สัตว์ทดลอง จึงมีความเจริญก้าวหน้าไปอย่างมาก ในการสร้าง embryonic stem cell นั้น จะกระทำได้โดย แยก inner cell mass ของเซลล์ตัวอ่อน แล้วนำไปเลี้ยงในอาหารเลี้ยงเชื้อที่มี fibroblast เป็น feeder cell หลังจากที่ได้ stem cell แล้ว ก็จะใช้เป็นต้นแบบในการศึกษาการแสดงออกของยีน และยังใช้เป็นต้นแบบในการสร้าง human embryonic stem cell ต่อไปในอนาคต
| 1. | Wilairat, P., Tirawanchai, N., Intapruk, C., Tungpradabkul, S., Sertsrivanich, R., Panyim, S., et al. Recombinant DNA techniques as potential diagnostic means. Ann Ist Super Sanita 1985;21(3):299-305. |
| 2. | Sinden, RE., Winger, L., Carter, EH., Hartley, RH., Tirawanchai, N., Davies, CS., et al. Ookinete antigens of Plasmodium berghei: a light and electron-microscope immunogold study of expression of the 21 kDa determinant recognized by a transmission-blocking antibody. Proc R Soc Lond B Biol Sci 1987;230(1261):443-58. |
| 3. | Tirawanchai, N., Winger, LA., Nicholas, J., Carter, HE., Smith, JE., Sinden, RE. Ookinete antigens of Plasmodium berghei. Appearance on the zygote surface of an Mr 21 kD determinant identified by transmission-blocking monoclonal antibodies. Parasite Immunol 1988;10(2):193-207. |
| 4. | Tirawanchai, N., Fucharoen, S., Wilairat, P., Panyim, S., Thaithong, S. Differentiation of Plasmodium falciparum clones by means of a repetitive DNA probe. Trans R Soc Trop Med Hyg 1988;82(2):209-11. |
| 5. | Tirawanchai, N., & Sinden, R. E. (1990). Three non-repeated transmission blocking epitopes recognized in the 21 kD surface antigen of zygotes-ookinetes of Plasmodium berghei. Parasite immunology, 12(4), 435–446. https://doi.org/10.1111/j.1365-3024.1990.tb00979.x |
| 6. | Sinden, RE., Tirawanchai, N., Fleck, S., Paton, M., Alejo-Blanco, R., Barker, G., et al. Molecular aspects of gametocyte differentiation. Geneva Switzerland 1990;90:3. |
| 7. | Sinden, RE., Tirawanchai, N., Fleck, S., Paton, M., Alejo-Blanco, R., Barker, G., et al. Transmission-blocking immunity in malaria : Characterisation of target antigens in the rodent. Geneva Switzerland 1990;90:3. |
| 8. | Tirawanchai, N., Winger, L. A., Nicholas, J., & Sinden, R. E. (1991). Analysis of immunity induced by the affinity-purified 21-kilodalton zygote-ookinete surface antigen of Plasmodium berghei. Infection and immunity, 59(1), 36–44. https://doi.org/10.1128/iai.59.1.36-44.1991 |
| 9. | Medley, G. F., Sinden, R. E., Fleck, S., Billingsley, P. F., Tirawanchai, N., & Rodriguez, M. H. (1993). Heterogeneity in patterns of malarial oocyst infections in the mosquito vector. Parasitology, 106 ( Pt 5), 441–449. https://doi.org/10.1017/s0031182000076721 |
| 10. | Tirawanchai, N., Ong-ajyooth, L., Ong-Ajyooth, S., Tobunluepop, P., Likidlilid, A., Nilwarangkul, S. Determination of the apolipoprotein E genotype using PCR-RFLP in kidney diseases. Siriraj Hosp Gaz 1995;47:1086-93. |
| 11. | Lekhakula, S., Angsusingha, K., Futrakul, A., Wacharasindhu, S., Likidmaskul, S., Tirawanchai, N., et al. Molecular studies in growth hormone gene deletions in patients with isolated growth hormone deficiency. Siriraj Hosp Gaz 1997;49:1048-53. |
| 12. | Lekhakula, S., Angsusingha, K., Futrakul, A., Wacharasindhu, S., Likidmaskul, S., Tirawanchai, N., et al. Studies of GH gene defects in familial isolated growth hormone deficiency (IGHD). Siriraj Hosp Gaz 1998;50:184-8. |
| 13. |
Tirawanchai, N., Yamwong, P., Pongrapeeporn, K. U., Likidlilid, A., Ong-Ajyooth, S., & Amornrattana, A. (2000). Screening for a D9N common mutation in exon 2 of the LPL gene in Thai normolipidemic and hyperlipidemic subjects. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 83 Suppl 2, S54–S60. |
| 14. |
Tirawanchai, N., Dulyasukdi, B., Likidlilid, A., Pongrapeeporn, K. U., Poldee, S., & Amornrattana, A. (2000). Polymorphism of the gene encoding lipoprotein lipase in thai primary hyperlipoproteinemias. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 83 Suppl 2, S19–S27. |
| 15. |
Weerakulwattana, L., Tirawanchai, N., & Bunyaratavej, N. (2001). Analysis of polymorphism of the interleukin-6 gene in Thai subjects with osteoporosis. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 84 Suppl 2, S547–S552. |
| 16. |
Peerapatdit, T. ., Likidlilid, . A. ., Patchanans, N. ., Tirawanchai, N. ., & Sriratanasathavorn, C. . (2004). Glutathione and Glutathione Peroxidase in Type 2 Diabetic Patients. Siriraj Medical Journal, 56(2), 53–62. retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/245485 |
| 17. |
Laohavechvanich, P., Kangsadalampai, K., Tirawanchai, N., & Ketterman, A. J. (2006). Effect of different Thai traditional processing of various hot chili peppers on urethane-induced somatic mutation and recombination in Drosophila melanogaster: assessment of the role of glutathione transferase activity. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 44(8), 1348–1354. https://doi.org/10.1016/j.fct.2006.02.013 |
| 18. |
Tirawanchai, N., Leowattana, W., Somkasetrin, A., & Thongsaen, P. (2008). Analysis of polymorphisms in the 5'-flanking region of the apolipoprotein(a) [Apo(a)] gene in Thai subjects with coronary artery diseases. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 91(1), 86–92. |
| 19. |
Tirawanchai, N., Somkasetrin, A., Avirutakarn, C., & Bunyaratvej, N. (2009). Determination of the Apo E genotype using LightCycler Apo E mutation detection kit. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 92 Suppl5, S81–S87. |
| 20. |
Phermthai, T., Suksompong, S., Tirawanchai, N., Issaragrisil, S., Julavijitphong, S., Wichitwiengrat, S., Silpsorn, D., & Pokathikorn, P. (2013). Epigenetic analysis and suitability of amniotic fluid stem cells for research and therapeutic purposes. Stem cells and development, 22(9), 1319–1328. https://doi.org/10.1089/scd.2012.0371 |
| 21. |
Julavijitphong, S., Wichitwiengrat, S., Tirawanchai, N., Ruangvutilert, P., Vantanasiri, C., & Phermthai, T. (2014). A xeno-free culture method that enhances Wharton's jelly mesenchymal stromal cell culture efficiency over traditional animal serum-supplemented cultures. Cytotherapy, 16(5), 683–691. https://doi.org/10.1016/j.jcyt.2013.07.012 |
| 22. |
Srettabunjong, S., Satitsri, S., Thongnoppakhun, W., & Tirawanchai, N. (2014). The study on telomere length for age estimation in a Thai population. The American journal of forensic medicine and pathology, 35(2), 148–153. https://doi.org/10.1097/PAF.0000000000000095 |
| 23. |
Phermthai, T., Thongbopit, S., Pokathikorn, P., Wichitwiengrat, S., Julavijitphong, S., & Tirawanchai, N. (2017). Carcinogenicity, efficiency and biosafety analysis in xeno-free human amniotic stem cells for regenerative medical therapies. Cytotherapy, 19(8), 990–1001. https://doi.org/10.1016/j.jcyt.2017.04.004 |
| 24. |
Tirawanchai, N., Supapornhemin, S., Somkasetrin, A., Suktitipat, B., & Ampawong, S. (2018). Regulatory effect of Phikud Navakot extract on HMG-CoA reductase and LDL-R: potential and alternate agents for lowering blood cholesterol. BMC complementary and alternative medicine, 18(1), 258. https://doi.org/10.1186/s12906-018-2327-1 |
| 25. |
Bumrungpert, A., Lilitchan, S., Tuntipopipat, S., Tirawanchai, N., & Komindr, S. (2018). Ferulic Acid Supplementation Improves Lipid Profiles, Oxidative Stress, and Inflammatory Status in Hyperlipidemic Subjects: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial. Nutrients, 10(6), 713. https://doi.org/10.3390/nu10060713 |
| 26. |
Pannangpetch, P., & Terashima, T. (2017). Lipid-lowering effect of Phyllanthus emblica and Alpinia galanga extracts on HepG2 cell line. Asian Pacific Journal of Tropical Biomedicine, 7(8), 694–697. https://doi.org/10.1016/j.apjtb.2017.07.006 |
| 27. |
Tirawanchai, N., Supapornhemin, S., Somkasetrin, A., Jantaravinid, J., Kengkoom, K., & Ampawong, S. (2019). Four Main Components of Phikud Navakot Promote Cholesterol Metabolism Through LDLR, HMGCR, SR-BI and ApoA-I Genes. Srinagarind Medical Journal, 34(5), 527–536. retrieved from https://li01.tci-thaijo.org/index.php/SRIMEDJ/article/view/217584 |
| 28. |
Tirawanchai, N., Kengkoom, K., Isarangkul, D., Burana-Osot, J., Kanjanapruthipong, T., Chantip, S., Phattanawasin, P., Sotanaphun, U., & Ampawong, S. (2020). A combination extract of kaffir lime, galangal, and lemongrass maintains blood lipid profiles, hepatocytes, and liver mitochondria in rats with nonalcoholic steatohepatitis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 124, 109843. https://doi.org/10.1016/j.biopha.2020.109843 |
| 29. |
Jantaravinid, J., Jiamton, S., Srisawat, C., Suktitipat, B., & Tirawanchai, N. (2021). Expression Pattern of Genes in Condyloma Acuminata Treated with Clinacanthus nutans Lindau Cream versus Podophyllin. Evidence-based complementary and alternative medicine : eCAM, 2021, 5579520. https://doi.org/10.1155/2021/5579520 |
| 30. |
Tuentam, K., Aramwit, P., Reamtong, O., Supasai, S., Chaisri, U., Fongsodsri, K., Yamdech, R., Tirawanchai, N., Sukphopetch, P., & Ampawong, S. (2023). Sericin-Based Poly(Vinyl) Alcohol Relieves Plaque and Epidermal Lesions in Psoriasis; a Chance for Dressing Development in a Specific Area. International Journal of Molecular Sciences, 24(1), 145. https://doi.org/10.3390/ijms24010145 |
| 31. |
Jantaravinid, J., Tirawanchai, N., Ampawong, S., Kengkoom, K., Somkasetrin, A., Nakhonsri, V., & Aramwit, P. (2024). Transcriptomic screening of novel targets of sericin in human hepatocellular carcinoma cells. Scientific reports, 14(1), 5455. https://doi.org/10.1038/s41598-024-56179-y |
| 32. |
Klaymook, S., Tirawanchai, N., Wichitwiengrat, S., Chuaynarong, P., Thongbopit, S., Chareancholvanich, K., & Phermthai, T. (2024). MSC secretome from amniotic fluid halts IL-1β and TNF-α inflammation via the ERK/MAPK pathway, promoting cartilage regeneration in OA in vitro. Journal of stem cells & regenerative medicine, 20(1), 3–13. https://doi.org/10.46582/jsrm.2001002 |
1.
Tirawanchai N, Phermthai T, Titapan V, Loakirkkiat P, Tongbopit S, Wichitwingrat S. Establishment of an in vitro culture of mesenchymal stem cell from human term placenta. Joint Conference in Medical Sciences 2011: Chula-Rama-Siriraj (JCMS2011), June 15-17, 2011 at IMPACT Exhibition and Convention Center, Thailand: 21.
2.
Tirawanchai N, Somkasetrin A, Chuanasa T, Meksuriyen D, Hokputsa S. Screening for lipid and glucose lowering effects in Thai medicinal plants. งานประชุมวิชาการประจำปี 2553 “โรงเรียนแพทย์ศิริราชเพื่อสังคม 120 ปีแห่งความก้าวหน้า” ระหว่างวันที่ 16 – 20 สิงหาคม 2553 ณ คณะแพทยศาสตร์ศิริราชพยาบาล มหาวิทยาลัยมหิดล
3.
Tirawanchai N, Somkasetrin A, Avirutakarn C, Bunyaratvej N. Characterisation of the ApoE genotypes in Thai post – menopausal subjects using LightCycler TM ApoE mutation detection kit. The 13th Vitamin K and Aging Symposium, February 18-22, 2009 at Tokyo, Japan.
4.
Tirawanchai N, Chanarat N, Somkasetrin A, Chanchairujira T, Srisawat C, Peerapittayamongkol C, et al. Study on the association of C677T and A1298C polymorphisms of the gene encoding methylenetetrahydrofolate reductase (MTHFR) enzyme and plasma homocysteine level in That hemodialysis patients. 33rd FEBS Congree & 11th IUBMB Conference, June 28-July 3, 2008 at Athens, Greece.
5.
Tirawanchai N, Thienprasert A, Somkasetrin A, Supasuwan P, Poldee S. Oxidative stress response in Thai school age children supplemented with omega-3 fatty acids. Presented in the 7th Congress of the International Society for the Study of Fatty Acids & Lipids (ISSFAL), July 23-28, 2006 at Cairns, Queensland, Australia.
6.
Somkasetrin A, Tirawanchai N, Bunyaratvej N, Avirutakarn C, Poldee S, Sopananurak P. Determination of the Apo E Genotype Using LightCycler? Apo E Mutation Detection Kit. Siriraj-Ramathibody Conference, April 18-21, 2006.
