{"id":4673,"date":"2025-12-17T15:36:32","date_gmt":"2025-12-17T08:36:32","guid":{"rendered":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/?p=4673"},"modified":"2026-03-30T10:33:28","modified_gmt":"2026-03-30T03:33:28","slug":"discovery-of-procyanidin-condensed-tannins-of-epicatechin-from-kratom-mitragyna-speciosa-as-virucidal-agents-against-sars-cov-2-2","status":"publish","type":"post","link":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/discovery-of-procyanidin-condensed-tannins-of-epicatechin-from-kratom-mitragyna-speciosa-as-virucidal-agents-against-sars-cov-2-2\/","title":{"rendered":"Discovery of procyanidin condensed tannins of (-)-epicatechin from Kratom, Mitragyna speciosa, as virucidal agents against SARS-CoV-2"},"content":{"rendered":"<p>Sanya Sureram, Nopporn Chutiwitoonchai, Tam Pooprasert, Watchara Sangsopha, Suphitcha Limjiasahapong, Narumol Jariyasopit, Yongyut Sirivatanauksorn, <strong> Sakda Khoomrung<\/strong>, Chulabhorn Mahidol, Somsak Ruchirawat, Prasat Kittakoop<\/p>\n<h2 class=\"title\">Abstract<\/h2>\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\n<div class=\"title\">\n<p>Kratom, Mitragyna speciosa, is one of the most popular herbs in the West and Southeast Asia. A number of previous works have focused on bioactive alkaloids in this plant; however, non-alkaloids have never been investigated for their biological activities. Antiviral and virucidal assays of a methanol leaf extract of Kratom, M. speciosa, revealed that a crude extract displayed virucidal activity against the SARS-CoV-2. Activity-guided isolation of a methanol leaf extract of Kratom led to the identification of B-type procyanidin condensed tannins of (-)-epicatechin as virucidal compounds against SARS-CoV-2. The fraction containing condensed tannins exhibited virucidal activity with an EC50 value of 8.38 \u03bcg\/mL and a selectivity index (SI) value &gt;23.86. LC-MS\/MS analysis and MALDI-TOF MS identified the structure of the virucidal compounds in Kratom as B-type procyanidin condensed tannins, while gel permeation chromatograph (GPC) revealed weight average molecular weight of 238,946 Da for high molecular-weight condensed tannins. In addition to alkaloids, (-)-epicatechin was found as a major component in the leaves of M. speciosa, but it did not have virucidal activity. Macromolecules of (-)-epicatechin, i.e., procyanidin condensed tannins, showed potent virucidal activity against SARS-CoV-2, suggesting that the high molecular weights of these polyphenols are important for virucidal activity.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/38866269\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/38866269\/<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Sanya Sureram, Nopporn Chutiwitoonchai, Tam Pooprasert, [&#8230;]\n","protected":false},"author":6,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-4673","post","type-post","status-publish","format-standard","hentry","category-3"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Discovery of procyanidin condensed tannins of (-)-epicatechin from Kratom, 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virucidal agents against SARS-CoV-2 - \u0e20\u0e32\u0e04\u0e27\u0e34\u0e0a\u0e32\u0e0a\u0e35\u0e27\u0e40\u0e04\u0e21\u0e35 \u0e04\u0e13\u0e30\u0e41\u0e1e\u0e17\u0e22\u0e28\u0e32\u0e2a\u0e15\u0e23\u0e4c\u0e28\u0e34\u0e23\u0e34\u0e23\u0e32\u0e0a\u0e1e\u0e22\u0e32\u0e1a\u0e32\u0e25 \u0e21\u0e2b\u0e32\u0e27\u0e34\u0e17\u0e22\u0e32\u0e25\u0e31\u0e22\u0e21\u0e2b\u0e34\u0e14\u0e25\" \/>\n<meta property=\"og:description\" content=\"Sanya Sureram, Nopporn Chutiwitoonchai, Tam Pooprasert, [...]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/discovery-of-procyanidin-condensed-tannins-of-epicatechin-from-kratom-mitragyna-speciosa-as-virucidal-agents-against-sars-cov-2-2\/\" \/>\n<meta property=\"og:site_name\" content=\"\u0e20\u0e32\u0e04\u0e27\u0e34\u0e0a\u0e32\u0e0a\u0e35\u0e27\u0e40\u0e04\u0e21\u0e35 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