{"id":4702,"date":"2025-12-17T15:53:22","date_gmt":"2025-12-17T08:53:22","guid":{"rendered":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/?p=4702"},"modified":"2026-03-30T10:18:59","modified_gmt":"2026-03-30T03:18:59","slug":"single-cell-rna-sequencing-reveals-the-expansion-of-circulating-tissue-homing-b-cell-subsets-in-secondary-acute-dengue-viral-infection-2","status":"publish","type":"post","link":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/single-cell-rna-sequencing-reveals-the-expansion-of-circulating-tissue-homing-b-cell-subsets-in-secondary-acute-dengue-viral-infection-2\/","title":{"rendered":"Single-cell RNA sequencing reveals the expansion of circulating tissue-homing B cell subsets in secondary acute dengue viral infection"},"content":{"rendered":"<p>Jantarika Kumar Arora, Ponpan Matangkasombut, <strong>Varodom Charoensawan<\/strong>, Anunya Opasawatchai; DENFREE Thailand<\/p>\n<p><strong>Collaborators<\/strong><br \/>\n<strong>DENFREE Thailand :<\/strong> Anavaj Sakuntabhai, Pratap Singhasivanon, Swangjit Suraamornkul, Tawatchai Yingtaweesak, Khajohnpong Manopwisedjaroen, Nada Pitabut<\/p>\n<p><h2 class=\"title\">Abstract<\/h2>\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\n<div class=\"title\">\n<p>The roles of antibodies secreted by subsets of B cells in dengue virus (DENV) infection have been extensively studied, yet, the contribution of tissue-homing B cells to antiviral immunity remains unclear. In this study, we performed a comprehensive analysis of B cell subpopulations in peripheral blood samples from DENV-infected patients using single-cell RNA-sequencing (scRNA-seq) datasets and flow cytometry. We showed that plasma cells (PCs) and plasmablasts (PBs) were the predominant B cell populations during the acute phase of secondary natural DENV infection, but not in convalescent phase nor in healthy controls. Interestingly, these cells expressed proliferation, adhesion, and tissue-homing genes, including SELPLG, a homing marker of the skin, the initial infected site of DENV. Flow cytometry analysis confirmed a significant upregulation of cell surface expression of a cutaneous lymphocyte-associated antigen (CLA) encoded by SELPLG in PCs and PBs, compared to naive and memory B cells from the same patients. The analysis of an independent single-cell B-cell receptor sequencing (scBCR-seq) dataset of DENV-infected patients revealed that the peripheral blood PCs and PBs exhibited the highest clonal expansion in secondary DENV infection compared to other B cell subsets. These clonally expanded cells also expressed the highest levels of tissue-homing genes, including SELPLG. In addition, by utilizing a public scRNA-seq dataset of SARS-CoV2 infection, we demonstrated the upregulation of several tissue-homing genes in PCs and PBs. Our study provides evidence for the potential roles of tissue-homing B cell subsets in the context of immune responses against viral infections in humans.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/38818157\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/38818157\/<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Jantarika Kumar Arora, Ponpan Matangkasombut, Varodom C [&#8230;]\n","protected":false},"author":6,"featured_media":4509,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-4702","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-3"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.0 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Single-cell RNA sequencing reveals the expansion of circulating tissue-homing B cell subsets in secondary acute dengue 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