{"id":5048,"date":"2025-12-19T11:48:50","date_gmt":"2025-12-19T04:48:50","guid":{"rendered":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/?p=5048"},"modified":"2026-03-25T11:51:53","modified_gmt":"2026-03-25T04:51:53","slug":"mass-spectrometry-based-analysis-of-gut-microbial-metabolites-of-aromatic-amino-acids","status":"publish","type":"post","link":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/mass-spectrometry-based-analysis-of-gut-microbial-metabolites-of-aromatic-amino-acids\/","title":{"rendered":"Mass spectrometry-based analysis of gut microbial metabolites of aromatic amino acids"},"content":{"rendered":"<p>Narumol Jariyasopit, <strong>Sakda Khoomrung<\/strong><\/p>\n<h2 class=\"title\">Abstract<\/h2>\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\n<div class=\"title\">\n<p>Small molecules derived from gut microbiota have been increasingly investigated to better understand the functional roles of the human gut microbiome. Microbial metabolites of aromatic amino acids (AAA) have been linked to many diseases, such as metabolic disorders, chronic kidney diseases, inflammatory bowel disease, diabetes, and cancer. Important microbial AAA metabolites are often discovered via global metabolite profiling of biological specimens collected from humans or animal models. Subsequent metabolite identity confirmation and absolute quantification using targeted analysis enable comparisons across different studies, which can lead to the establishment of threshold concentrations of potential metabolite biomarkers. Owing to their excellent selectivity and sensitivity, hyphenated mass spectrometry (MS) techniques are often employed to identify and quantify AAA metabolites in various biological matrices. Here, we summarize the developments over the past five years in MS-based methodology for analyzing gut microbiota-derived AAA. Sample preparation, method validation, analytical performance, and statistical methods for correlation analysis are discussed, along with future perspectives.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37841334\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/37841334\/<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Narumol Jariyasopit, Sakda Khoomrung Abstract Small mol [&#8230;]\n","protected":false},"author":6,"featured_media":5050,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-5048","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-3"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Mass spectrometry-based analysis of gut microbial metabolites of aromatic amino acids - 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