{"id":6086,"date":"2026-08-19T13:57:24","date_gmt":"2026-08-19T06:57:24","guid":{"rendered":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/?p=6086"},"modified":"2026-08-19T14:00:39","modified_gmt":"2026-08-19T07:00:39","slug":"genetic-correction-of-haemoglobin-e-in-an-immortalised-haemoglobin-e-beta-thalassaemia-cell-line-using-the-crispr-cas9-system","status":"publish","type":"post","link":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/genetic-correction-of-haemoglobin-e-in-an-immortalised-haemoglobin-e-beta-thalassaemia-cell-line-using-the-crispr-cas9-system\/","title":{"rendered":"Genetic correction of haemoglobin E in an immortalised haemoglobin E\/beta-thalassaemia cell line using the CRISPR\/Cas9 system"},"content":{"rendered":"<p><strong>Kongtana Trakarnsanga<\/strong>, Nontaphat Thongsin, <strong>Chanatip Metheetrairut<\/strong>, Chartsiam Tipgomut, <strong>Saiphon Poldee<\/strong>, Methichit Wattanapanitch<\/p>\n<h2 class=\"title\">Abstract<\/h2>\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\n<div class=\"title\">\n<p>\u03b2-thalassaemia is one of the most common genetic blood diseases worldwide with over 300 mutations in the HBB gene affecting red blood cell functions. Recently, advances in genome editing technology have provided a powerful tool for precise genetic correction. Generation of patient-derived induced pluripotent stem cells (iPSCs) followed by genetic correction of HBB mutations and differentiation into haematopoietic stem\/progenitor cells (HSPCs) offers a potential therapy to cure the disease. However, the biggest challenge is to generate functional HSPCs that are capable of self-renewal and transplantable. In addition, functional analyses of iPSC-derived erythroid cells are hampered by poor erythroid expansion and incomplete erythroid differentiation. Previously, we generated an immortalised erythroid cell line (SiBBE) with unique properties, including unlimited expansion and the ability to differentiate into mature erythrocytes. In this study, we report a highly efficient genetic correction of HbE mutation in the SiBBE cells using the CRISPR\/Cas9 system. The HbE-corrected clones restored \u03b2-globin production with reduced levels of HbE upon erythroid differentiation. Our approach provides a sustainable supply of corrected erythroid cells and represents a valuable model for validating the therapeutic efficacy of gene editing systems.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36114353\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/36114353\/<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Kongtana Trakarnsanga, Nontaphat Thongsin, Chanatip Met [&#8230;]\n","protected":false},"author":6,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-6086","post","type-post","status-publish","format-standard","hentry","category-3"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Genetic correction of haemoglobin E in an immortalised haemoglobin E\/beta-thalassaemia cell line using the CRISPR\/Cas9 system - 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