{"id":6155,"date":"2026-08-20T13:32:26","date_gmt":"2026-08-20T06:32:26","guid":{"rendered":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/?p=6155"},"modified":"2026-08-20T13:34:14","modified_gmt":"2026-08-20T06:34:14","slug":"methods-for-mitochondrial-health-assessment-by-high-content-imaging-system","status":"publish","type":"post","link":"https:\/\/www2.si.mahidol.ac.th\/department\/biochemistry\/methods-for-mitochondrial-health-assessment-by-high-content-imaging-system\/","title":{"rendered":"Methods for mitochondrial health assessment by High Content Imaging System"},"content":{"rendered":"<p>Chatnapa Panusatid, Nattachai Thangsiriskul, <strong>Chayanon Peerapittayamongkol<\/strong><\/p>\n<h2 class=\"title\">Abstract<\/h2>\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\n<div class=\"title\">\n<p>Mitochondria are important organelles responsible for energy production. Mitochondrial dysfunction relates to various pathological diseases. The investigation of mitochondrial heath is critical to evaluate the cellular status. Herein, we demonstrated an approach for determining the status of mitochondrial health by observing mitochondrial H2O2 (one type of ROS), membrane potential, and morphology (fragmentation and length) in live primary fibroblast cells. The cells were co-stained with fluorescent dyes (Hoechst 33342 and MITO-ID\u00ae Red\/MitoPY1\/JC-10) and continuously processed by the High Content Imaging System. We employed the Operetta CLSTM to take fluorescent images with its given quickness and high resolution. The CellProfiler image analysis software was further used to identify cell and mitochondrial phenotypes in the thousand fluorescent images.\u2022We could quantitatively analyze fluorescent images with high-throughput and high-speed detection to track the alteration of mitochondrial status.\u2022The MMP assay is sensitive to FCCP even at the concentration of 0.01 \u00b5M.\u2022The fibroblast cells treated with stress inducers (H2O2, FCCP, and phenanthroline) revealed a significant change in mitochondrial health parameters, with more ROS accumulation, depolarized MMP, increased fragmentation, and reduced length of mitochondria.<\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35464807\/\">https:\/\/pubmed.ncbi.nlm.nih.gov\/35464807\/<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Chatnapa Panusatid, Nattachai Thangsiriskul, Chayanon P [&#8230;]\n","protected":false},"author":6,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-6155","post","type-post","status-publish","format-standard","hentry","category-3"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Methods for mitochondrial health assessment by High Content Imaging System - \u0e20\u0e32\u0e04\u0e27\u0e34\u0e0a\u0e32\u0e0a\u0e35\u0e27\u0e40\u0e04\u0e21\u0e35 \u0e04\u0e13\u0e30\u0e41\u0e1e\u0e17\u0e22\u0e28\u0e32\u0e2a\u0e15\u0e23\u0e4c\u0e28\u0e34\u0e23\u0e34\u0e23\u0e32\u0e0a\u0e1e\u0e22\u0e32\u0e1a\u0e32\u0e25 \u0e21\u0e2b\u0e32\u0e27\u0e34\u0e17\u0e22\u0e32\u0e25\u0e31\u0e22\u0e21\u0e2b\u0e34\u0e14\u0e25<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" 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