Author Archives: Poorintra Sakuna

Validation of a Thai semiquantitative food frequency questionnaire (semi-FFQ) for people at risk of metabolic syndrome

Nattvara Nirdnoy, Kitti Sranacharoenpong, Apinya Surawit, Bonggochpass Pinsawas, Pichanun Mongkolsucharitkul, Tanyaporn Pongkunakorn, Thamonwan Manosan, Suphawan Ophakas, Sophida Suta, Sureeporn Pumeiam, Korapat Mayurasakorn; Siriraj Health Study Group Collaborators Siriraj Health Study Group : Winai Ratanasuwan, Mayuree Homsanit, Keerati Charoencholvanich, Bhoom Suktitipat, Chalermchai Mitrpant, Manop Pithukpakorn, Prapat Suriyaphol, Rungroj Krittayaphong, Prasert Auewarakul, Boonrat Tassaneetritap, Naravat Poungvarin Abstract […]

Deep learning facilitates multi-data type analysis and predictive biomarker discovery in cancer precision medicine

Vivek Bhakta Mathema, Partho Sen, Santosh Lamichhane, Matej Orešič, Sakda Khoomrung Abstract Cancer progression is linked to gene-environment interactions that alter cellular homeostasis. The use of biomarkers as early indicators of disease manifestation and progression can substantially improve diagnosis and treatment. Large omics datasets generated by high-throughput profiling technologies, such as microarrays, RNA sequencing, whole-genome […]

Revisiting chloroplast genomic landscape and annotation towards comparative chloroplast genomes of Rhamnaceae

Kwanjeera Wanichthanarak, Intawat Nookaew, Phongthana Pasookhush, Thidathip Wongsurawat, Piroon Jenjaroenpun, Namkhang Leeratsuwan, Songsak Wattanachaisaereekul, Wonnop Visessanguan, Yongyut Sirivatanauksorn, Narong Nuntasaen, Chutima Kuhakarn, Vichai Reutrakul, Pravech Ajawatanawong, Sakda Khoomrung Abstract Background: Massive parallel sequencing technologies have enabled the elucidation of plant phylogenetic relationships from chloroplast genomes at a high pace. These include members of the family […]

iPSC-based approach for human hair follicle regeneration

Chinnavuth Vatanashevanopakorn, Thanutchaporn Sartyoungkul Abstract Hair follicles (HFs) are a multifunctional structure involved in physical protection, thermoregulation, sensational detection, and wound healing. Formation and cycling of HFs require dynamic interaction between different cell types of the follicles. Although the processes have been well studied, the generation of human functional HFs with a normal cycling pattern […]

Electrochemical aptasensor detection of electron transfer flavoprotein subunit beta for leptospirosis diagnosis

Uraiwan Kositanont, Chatchawan Srisawat, Sirinapa Sripinitchai, Charin Thawornkuno, Thanyarat Chaibun, Sinthu Karunaithas, Chamras Promptmas, Benchaporn Lertanantawong Abstract Electron transfer flavoprotein subunit beta (ETFB) of Leptospira interrogans is a biomarker for diagnosing leptospiral infection. Thus, the ETFB-specific nuclease-resistant RNA aptamer ETFB3-63 was developed and used in an electrochemical aptasensor to assay ETFB. Although the majority of […]

Exploiting nanopore sequencing for characterization and grading of IDH-mutant gliomas

Thidathip Wongsurawat, Piroon Jenjaroenpun, Panatna Anekwiang, Tantip Arigul, Wichayapat Thongrattana, Azemat Jamshidi-Parsian, Gunnar Boysen, Prapat Suriyaphol, Bhoom Suktitipat, Prajak Srirabheebhat, Pornsuk Cheunsuchon, Jantima Tanboon, Intawat Nookaew, Sith Sathornsumetee Abstract The 2021 WHO Classification of Central Nervous System Tumors recommended evaluation of cyclin-dependent kinase inhibitor 2A/B (CDKN2A/B) deletion in addition to codeletion of 1p/19q to characterize […]

Mass spectrometry-based analysis of gut microbial metabolites of aromatic amino acids

Narumol Jariyasopit, Sakda Khoomrung Abstract Small molecules derived from gut microbiota have been increasingly investigated to better understand the functional roles of the human gut microbiome. Microbial metabolites of aromatic amino acids (AAA) have been linked to many diseases, such as metabolic disorders, chronic kidney diseases, inflammatory bowel disease, diabetes, and cancer. Important microbial AAA […]

Influence of Prolonged Whole Egg Supplementation on Insulin-like Growth Factor 1 and Short-Chain Fatty Acids Product: Implications for Human Health and Gut Microbiota

Sophida Suta, Suphawan Ophakas, Thamonwan Manosan, Orranich Honwichit, Suvimol Charoensiddhi, Apinya Surawit, Tanyaporn Pongkunakorn, Sureeporn Pumeiam, Pichanun Mongkolsucharitkul, Bonggochpass Pinsawas, Sawannee Sutheeworapong, Patcha Puangsombat, Sakda Khoomrung, Korapat Mayurasakorn Abstract The gut microbiota exert a profound influence on human health and metabolism, with microbial metabolites playing a pivotal role in shaping host physiology. This study investigated […]

Anti-Xanthine Oxidase 5′-Hydroxyhericenes A-D from the Edible Mushroom Hericium erinaceus and Structure Revision of 3-[2,3-Dihydroxy-4-(hydroxymethyl)tetrahydrofuran-1-yl]-pyridine-4,5-diol

Tawatchai Thongkongkaew, Narumol Jariyasopit, Sakda Khoomrung, Siraprapa Siritutsoontorn, Sarawut Jitrapakdee, Prasat Kittakoop, Somsak Ruchirawat Abstract Hericium erinaceus is an edible mushroom with diverse pharmaceutical applications. Although this mushroom is an attractive source of natural products for cancer treatment, little is known about the bioactive compounds from this mushroom, which may possess antibreast cancer activity. Here, […]

Characterizing Different Modes of Interplay Between Rap1 and H3 Using Inducible H3-depletion Yeast

Peerapat Khamwachirapithak, David Guillaume-Schoepfer, Pakkanan Chansongkrow, Sarah A Teichmann, Philip A Wigge, Varodom Charoensawan Abstract Histones and transcription factors (TFs) are two important DNA-binding proteins that interact, compete, and together regulate transcriptional processes in response to diverse internal and external stimuli. Condition-specific depletion of histones in Saccharomyces cerevisiae using a galactose-inducible H3 promoter provides a […]