Asst. Prof. Chalermchai Mitrpant, MD, PhD

  • M.D. Faculty of Medicine Siriraj Hospital, Mahidol University
  • Ph.D. (Human Biology), Edith Cowan University, Australia

Office : 1009 Srisavarindira Bldg. 10th Fl. Department of Biochemistry. Faculty of Medicine, Siriraj Hospital, Bangkoknoi, Bangkok 10700

Laboratory : 924 Srisavarindira Bldg. 9rd Fl.

Phone : (02) 419-7585

E-Mail : chalermchai.mit@mahidol.edu

Current appointment
  • Assistant Professor, Faculty of Medicine, Siriraj Hospital, Mahidol University
  • Adjunct senior Lecturer, Murdoch University, Australia.
  • International Affiliate, Perron Institute for Neurological and Translational Science, Perth, Nedlands, Perth, Western Australia, Australia.
Research Profile

We have commenced our research on antisense oligomer (AO) mediated splice switching (viz. exon switching and exon inclusion) in neuromuscular disorders; i.e. Duchenne muscular dystrophy (DMD) and spinal muscular atrophy (SMA): Subsequently, our interests are expanded to cellular metabolism and cancer biology. Recently, zebrafish Facility for Biomedical Research (ZeBR) has been developed and utilised to study cancer biology and erythropoiesis.

Chalermchai Mitrpant

Principal Investigator

Kannika Siripattarapravat

Consultant

Raveevatoo Buathong, Ph.D.

Researcher

Jarichad Toosaranont

Research Assistant

Soraya Kalayanamontri

Research Assistant

Narisa Poonsub

Research Assistant

Nawarat Boonmee

Research Assistant

Natsamon Singha-art

Research officer

Saowanee Swangkaew

Research officer

Sunisa Kaewmee

Research officer

Pavita Matarach

Scientist
(Project coordinator)

Wipada Phromkhunthod

Part-time Research

  • Ei Ei Phyoe Mint

Master student (2012-2015)

  • Pichsinee Boonchuay

Master student (2012-2015)

  • Htoo Pyei Hlaing

Master degree Student (2014-2017)

  • Nawarat Boonmee

Master degree Student (2017-2020)

  • Pawarit Innachai

Master degree Student (2017-2021)

  • Sasiwimon Utama

PhD. Candidate (2016-2020)

  • Janya Grainok

PhD. Candidate (2016-2020)

  • Khin Kay Zin Tun

PhD. Student (2013-2017)

  • Nalinee Pradubyat
  • Kajol Thapa
  • Khine Zaw

PhD. Candidate Joint PhD. Programme in Biomedical Sciences
and Biotechnology (Liverpool, UK/CU) (2019)

Master degree Student (2022)

PhD. Student (2023)

1. Senanarong, V., Harnphadungkit, K., Lertrit, P., Mitrpant, C., Udompunthurak, S., Limwong, C., Prayoonwiwat, N., & Poungvarin, N. (2001). Experience of ApoE study in Thai elderly. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 84(2), 182–187.
2. Mitrpant, C., Fletcher, S., & Wilton, S. D. (2009). Personalised genetic intervention for Duchenne muscular dystrophy: antisense oligomers and exon skipping. Current molecular pharmacology, 2(1), 110–121. https://doi.org/10.2174/1874467210902010110
3. Mitrpant, C., Fletcher, S., Iversen, P. L., & Wilton, S. D. (2009). By-passing the nonsense mutation in the 4 CV mouse model of muscular dystrophy by induced exon skipping. The journal of gene medicine, 11(1), 46–56. https://doi.org/10.1002/jgm.1265
4. Mitrpant, C., Adams, A. M., Meloni, P. L., Muntoni, F., Fletcher, S., & Wilton, S. D. (2009). Rational design of antisense oligomers to induce dystrophin exon skipping. Molecular therapy : the journal of the American Society of Gene Therapy, 17(8), 1418–1426. https://doi.org/10.1038/mt.2009.49
5. Ferreira, M. A., Matheson, M. C., Duffy, D. L., Marks, G. B., Hui, J., Le Souëf, P., Danoy, P., Baltic, S., Nyholt, D. R., Jenkins, M., Hayden, C., Willemsen, G., Ang, W., Kuokkanen, M., Beilby, J., Cheah, F., de Geus, E. J., Ramasamy, A., Vedantam, S., Salomaa, V., … Australian Asthma Genetics Consortium (2011). Identification of IL6R and chromosome 11q13.5 as risk loci for asthma. Lancet (London, England), 378(9795), 1006–1014. https://doi.org/10.1016/S0140-6736(11)60874-X
6. Anderton, R. S., Price, L. L., Turner, B. J., Meloni, B. P., Mitrpant, C., Mastaglia, F. L., Goh, C., Wilton, S. D., & Boulos, S. (2012). Co-regulation of survival of motor neuron and Bcl-xL expression: implications for neuroprotection in spinal muscular atrophy. Neuroscience, 220, 228–236. https://doi.org/10.1016/j.neuroscience.2012.06.042
7. Porensky, P. N., Mitrpant, C., McGovern, V. L., Bevan, A. K., Foust, K. D., Kaspar, B. K., Wilton, S. D., & Burghes, A. H. (2012). A single administration of morpholino antisense oligomer rescues spinal muscular atrophy in mouse. Human molecular genetics, 21(7), 1625–1638. https://doi.org/10.1093/hmg/ddr600
8. Rojana-udomsart, A., Mitrpant, C., Bundell, C., Price, L., Luo, Y. B., Fabian, V., Wilton, S. D., Hollingsworth, P., & Mastaglia, F. L. (2013). Complement-mediated muscle cell lysis: a possible mechanism of myonecrosis in anti-SRP associated necrotizing myopathy (ASANM). Journal of neuroimmunology, 264(1-2), 65–70. https://doi.org/10.1016/j.jneuroim.2013.08.008
9. Luo, Y. B., Mitrpant, C., Johnsen, R., Fabian, V., Needham, M., Fletcher, S., Wilton, S. D., & Mastaglia, F. L. (2013). Investigation of splicing changes and post-translational processing of LMNA in sporadic inclusion body myositis. International Journal of Clinical and Experimental Pathology, 6(9), 1723-1733.
10. Zhou, H., Janghra, N., Mitrpant, C., Dickinson, R. L., Anthony, K., Price, L., Eperon, I. C., Wilton, S. D., Morgan, J., & Muntoni, F. (2013). A novel morpholino oligomer targeting ISS-N1 improves rescue of severe spinal muscular atrophy transgenic mice. Human gene therapy, 24(3), 331–342. https://doi.org/10.1089/hum.2012.211
11. Rojana-udomsart, A., Mitrpant, C., James, I., Witt, C., Needham, M., Day, T., Kiers, L., Corbett, A., Martinez, P., Wilton, S. D., & Mastaglia, F. L. (2013). Analysis of HLA-DRB3 alleles and supertypical genotypes in the MHC Class II region in sporadic inclusion body myositis. Journal of neuroimmunology, 254(1-2), 174–177. https://doi.org/10.1016/j.jneuroim.2012.09.003
12. Luo, Y. B., Mitrpant, C., Johnsen, R. D., Fabian, V. A., Fletcher, S., Mastaglia, F. L., & Wilton, S. D. (2013). Investigation of age-related changes in LMNA splicing and expression of progerin in human skeletal muscles. International Journal of Clinical and Experimental Pathology, 6(12), 2778-2786.
13. Mitrpant, C., Porensky, P., Zhou, H., Price, L., Muntoni, F., Fletcher, S., Wilton, S. D., & Burghes, A. H. (2013). Improved antisense oligonucleotide design to suppress aberrant SMN2 gene transcript processing: towards a treatment for spinal muscular atrophy. PloS one, 8(4), e62114. https://doi.org/10.1371/journal.pone.0062114
14. Luo, Y. B., Mitrpant, C., Adams, A. M., Johnsen, R. D., Fletcher, S., Mastaglia, F. L., & Wilton, S. D. (2014). Antisense oligonucleotide induction of progerin in human myogenic cells. PloS one, 9(6), e98306. https://doi.org/10.1371/journal.pone.0098306
15. Sitticharoon, C., Boonpuan, V., Mitrpant, C., & Churintaraphan, M. (2017). Determination of KISS1, KISS1R and Kisspeptin in Fat Tissue of Normal Weight and Obese Humans and Correlations between Serum Kisspeptin and Leptin. Siriraj Medical Journal, 65(4), 112–116. retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/82333
16. Flynn, L. L., Mitrpant, C., Pitout, I. L., Fletcher, S., & Wilton, S. D. (2018). Antisense Oligonucleotide-Mediated Terminal Intron Retention of the SMN2 Transcript. Molecular therapy. Nucleic acids, 11, 91–102. https://doi.org/10.1016/j.omtn.2018.01.011
17. Zaw, K., Greer, K., Aung-Htut, M. T., Mitrpant, C., Veedu, R. N., Fletcher, S., & Wilton, S. D. (2019). Consequences of Making the Inactive Active Through Changes in Antisense Oligonucleotide Chemistries. Frontiers in genetics, 10, 1249. https://doi.org/10.3389/fgene.2019.01249
18. Zaw, K., Wong, E. Y. M., Zhang, X., Zhang, D., Chen, S. C., Thompson, J. A., Lamey, T., McLaren, T., De Roach, J. N., Wilton, S. D., Fletcher, S., Mitrpant, C., Atlas, M. D., Chen, F. K., & McLenachan, S. (2021). Generation of three induced pluripotent stem cell lines from a patient with Usher syndrome caused by biallelic c.949C > A and c.1256G > T mutations in the USH2A gene. Stem Cell Research, 50, Article 102129. https://doi.org/10.1016/j.scr.2020.102129
19. Pharkjaksu, S., Chongtrakool, P., Chayakulkeeree, M., Mitrpant, C., Angkasekwinai, P., Bennett, J. E., Kwon-Chung, K. J., & Ngamskulrungroj, P. (2020). Cryptococcus neoformans/gattii Species Complexes from Pre-HIV Pandemic Era Contain Unusually High Rate of Non-Wild-Type Isolates for Amphotericin B. Infection and drug resistance, 13, 673–681. https://doi.org/10.2147/IDR.S235473
20. Tipgomut, C., Khuhapinant, A., Wilson, M. C., Poldee, S., Heesom, K. J., Metheetrairut, C., Sripichai, O., Mitrpant, C., Frayne, J., & Trakarnsanga, K. (2021). MTAP-related increased erythroblast proliferation as a mechanism of polycythaemia vera. Scientific reports, 11(1), 22483. https://doi.org/10.1038/s41598-021-01877-0
21. Pharkjaksu, S., Boonmee, N., Mitrpant, C., & Ngamskulrungroj, P. (2021). Immunopathogenesis of Emerging Candida auris and Candida haemulonii Strains. Journal of fungi (Basel, Switzerland), 7(9), 725. https://doi.org/10.3390/jof7090725
22. Flynn, L. L., Mitrpant, C., Adams, A., Pitout, I. L., Stirnweiss, A., Fletcher, S., & Wilton, S. D. (2021). Targeted SMN Exon Skipping: A Useful Control to Assess In Vitro and In Vivo Splice-Switching Studies. Biomedicines, 9(5), 552. https://doi.org/10.3390/biomedicines9050552
23. Oo, K. K., Kamolhan, T., Soni, A., Thongchot, S., Mitrpant, C., O-Charoenrat, P., Thuwajit, C., & Thuwajit, P. (2021). Development of an engineered peptide antagonist against periostin to overcome doxorubicin resistance in breast cancer. BMC cancer, 21(1), 65. https://doi.org/10.1186/s12885-020-07761-w
24. Pokhrel, S., Boonmee, N., Tulyaprawat, O., Pharkjaksu, S., Thaipisutikul, I., Chairatana, P., Ngamskulrungroj, P., & Mitrpant, C. (2022). Assessment of Biofilm Formation by Candida albicans Strains Isolated from Hemocultures and Their Role in Pathogenesis in the Zebrafish Model. Journal of fungi (Basel, Switzerland), 8(10), 1014. https://doi.org/10.3390/jof8101014
25. Chuenkongkaew, W. L., Chinkulkitnivat, B., Lertrit, P., Chirapapaisan, N., Kaewsutthi, S., Suktitipat, B., & Mitrpant, C. (2022). Clinical expression and mitochondrial deoxyribonucleic acid study in twins with 14484 Leber's hereditary optic neuropathy: A case report. World journal of clinical cases, 10(20), 6944–6953. https://doi.org/10.12998/wjcc.v10.i20.6944
26. Toosaranont, J., Ruschadaariyachat, S., Mujchariyakul, W., Arora, J. K., Charoensawan, V., Suktitipat, B., Palmer, T. N., Fletcher, S., Wilton, S. D., & Mitrpant, C. (2022). Antisense Oligonucleotide Induction of the hnRNPA1b Isoform Affects Pre-mRNA Splicing of SMN2 in SMA Type I Fibroblasts. International journal of molecular sciences, 23(7), 3937. https://doi.org/10.3390/ijms23073937
27. Tongkaew, P., Tohraman, A., Bungaramphai, R., Mitrpant, C., & Aydin, E. (2022). Kluai Hin (Musa sapientum Linn.) peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function. Scientific reports, 12(1), 4145. https://doi.org/10.1038/s41598-022-08008-3
28. Pradubyat, N., Giannoudis, A., Elmetwali, T., Mahalapbutr, P., Palmieri, C., Mitrpant, C., & Ketchart, W. (2022). 1'-Acetoxychavicol Acetate from Alpinia galanga Represses Proliferation and Invasion, and Induces Apoptosis via HER2-signaling in Endocrine-Resistant Breast Cancer Cells. Planta medica, 88(2), 163–178. https://doi.org/10.1055/a-1307-3997
29. Nirdnoy, N., Sranacharoenpong, K., Surawit, A., Pinsawas, B., Mongkolsucharitkul, P., Pongkunakorn, T., Manosan, T., Ophakas, S., Suta, S., Pumeiam, S., Mayurasakorn, K., & Siriraj Health Study Group (2023). Validation of a Thai semiquantitative food frequency questionnaire (semi-FFQ) for people at risk of metabolic syndrome. Journal of health, population, and nutrition, 42(1), 13. https://doi.org/10.1186/s41043-023-00353-x
30. Utama, S., Cale, J. M., Mitrpant, C., Fletcher, S., Wilton, S. D., & Aung-Htut, M. T. (2024). Is Exon Skipping a Viable Therapeutic Approach for Vascular Ehlers-Danlos Syndrome with Mutations in COL3A1 Exon 10 or 15?. International journal of molecular sciences, 25(16), 8816. https://doi.org/10.3390/ijms25168816
31. Grainok, J., Pitout, I. L., Chen, F. K., McLenachan, S., Heath Jeffery, R. C., Mitrpant, C., & Fletcher, S. (2024). A Precision Therapy Approach for Retinitis Pigmentosa 11 Using Splice-Switching Antisense Oligonucleotides to Restore the Open Reading Frame of PRPF31. International journal of molecular sciences, 25(6), 3391. https://doi.org/10.3390/ijms25063391
32. Opassathian, K., Boonmee, N., Swangkaew, S., Jenjaroenpun, P., Wongsurawat, T., Kruasuwan, W., Ngamskulrungroj, P., & Mitrpant, C. (2025). A study of genomic complexity underlying multidrug resistance in Candida auris strains in Thailand.
Scientific Reports, 15, Article 41478. https://doi.org/10.1038/s41598-025-25386-6