Asst. Prof. Chanatip Metheetrairut, PhD

  • B.S. (Phi Beta Kappa, magna cum laude, Distinction in the Major), Molecular Biophysics & Biochemistry, Yale University, New Haven, CT, USA.
  • Ph.D., Molecular, Cellular and Developmental Biology, Mentor: Frank J. Slack, Yale University, New Haven, CT, USA

Office : 1025 Srisavarindira Bldg. 10th Fl. Department of Biochemistry. Faculty of Medicine, Siriraj Hospital , Bangkoknoi, Bangkok 10700

Laboratory : 910 Srisavarindira Bldg. 9rd Fl.

Phone : (02) 419-9145

E-Mail : chanatip.met@mahidol.ac.th

Research

microRNAs (miRNAs) are endogenous small regulatory RNAs (~19-22nt) found in a wide range of organisms, including humans. They function as part of the post-transcriptional regulation of genes through binding with imperfect complementary sites in the 3′ UTRs of their target mRNAs and generally downregulating them. My research interest is in identifying the functions of specific miRNAs in different processes with the overall goal that these small RNAs may act to modulate some specific phenotypes or serve as biomarkers in diseases.

I have worked on the roles of miRNAs in sensitizing cells to or protecting cells from gamma radiation, utilizing the nematode, C. elegans, and breast cancer cell lines as models.

1. Metheetrairut, C., & Slack, F. J. (2013). MicroRNAs in the ionizing radiation response and in radiotherapy. Current opinion in genetics & development, 23(1), 12–19. https://doi.org/10.1016/j.gde.2013.01.002
2. Abraham, M. C., Metheetrairut, C., & Irish, V. F. (2013). Natural variation identifies multiple loci controlling petal shape and size in Arabidopsis thaliana. PloS one, 8(2), e56743. https://doi.org/10.1371/journal.pone.0056743
3. Coira, I. F., Rufino-Palomares, E. E., Romero, O. A., Peinado, P., Metheetrairut, C., Boyero-Corral, L., Carretero, J., Farez-Vidal, E., Cuadros, M., Reyes-Zurita, F. J., Lupiáñez, J. A., Sánchez-Cespedes, M., Slack, F. J., & Medina, P. P. (2015). Expression inactivation of SMARCA4 by microRNAs in lung tumors. Human molecular genetics, 24(5), 1400–1409. https://doi.org/10.1093/hmg/ddu554
4. Metheetrairut, C., Adams, B. D., Nallur, S., Weidhaas, J. B., & Slack, F. J. (2017). cel-mir-237 and its homologue, hsa-miR-125b, modulate the cellular response to ionizing radiation. Oncogene, 36(4), 512–524. https://doi.org/10.1038/onc.2016.222
5. Salzman, D. W., Nakamura, K., Nallur, S., Dookwah, M. T., Metheetrairut, C. , Slack, F. J., & Weidhaas, J. B. (2016). miR-34 activity is modulated through 5'-end phosphorylation in response to DNA damage. Nature communications, 7, 10954. https://doi.org/10.1038/ncomms10954
6. Metheetrairut, C. Ahuja, Y., & Slack, F. J. (2017). acn-1, a C. elegans homologue of ACE, genetically interacts with the let-7 microRNA and other heterochronic genes. Cell cycle (Georgetown, Tex.), 16(19), 1800–1809. https://doi.org/10.1080/15384101.2017.1344798
7. Poldee, S., Metheetrairut, C., Nugoolsuksiri, S., Frayne, J., & Trakarnsanga, K. (2018). Optimization of an erythroid culture system to reduce the cost of in vitro production of red blood cells. MethodsX, 5, 1626–1632. https://doi.org/10.1016/j.mex.2018.11.018
8. Kitdumrongthum, S., Metheetrairut, C., Charoensawan, V., Ounjai, P., Janpipatkul, K., Panvongsa, W., Weerachayaphorn, J., Piyachaturawat, P., & Chairoungdua, A. (2018). Dysregulated microRNA expression profiles in cholangiocarcinoma cell-derived exosomes.Life sciences, 210, 65–75. https://doi.org/10.1016/j.lfs.2018.08.058
9. Chuaijit, S., Boonyatistan, W., Boonchuay, P., Metheetrairut, C., & Suthammarak, W. (2019). Identification of a novel mitochondrial complex I assembly factor ACDH-12 in Caenorhabditis elegans. Mitochondrion, 46, 91–96. https://doi.org/10.1016/j.mito.2018.02.010
10. Metheetrairut, C., Chotigavanich, C., Amornpichetkul, K., Keskool, P., Ongard, S., & Metheetrairut, C. (2019). Expression levels of miR-34-family microRNAs are associated with TP53 mutation status in head and neck squamous cell carcinoma. European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 276(2), 521–533. https://doi.org/10.1007/s00405-018-5223-x
11. Trakarnsanga, K., Tipgomut, C., Metheetrairut, C., Wattanapanitch, M., Khuhapinant, A., Poldee, S., Kurita, R., Nakamura, Y., Srisawat, C., & Frayne, J. (2020). Generation of an immortalised erythroid cell line from haematopoietic stem cells of a haemoglobin E/β-thalassemia patient. Scientific reports, 10(1), 16798. https://doi.org/10.1038/s41598-020-73991-4
12. Tipgomut, C., Khuhapinant, A., Wilson, M. C., Poldee, S., Heesom, K. J., Metheetrairut, C., Sripichai, O., Mitrpant, C., Frayne, J., & Trakarnsanga, K. (2021). MTAP-related increased erythroblast proliferation as a mechanism of polycythaemia vera. Scientific reports, 11(1), 22483. https://doi.org/10.1038/s41598-021-01877-0
13. Trakarnsanga, K., Thongsin, N.,Metheetrairut, C.,Tipgomut, C., Poldee, S., & Wattanapanitch, M. (2022). Genetic correction of haemoglobin E in an immortalised haemoglobin E/beta-thalassaemia cell line using the CRISPR/Cas9 system. Scientific reports, 12(1), 15551. https://doi.org/10.1038/s41598-022-19934-7
14. Metheetrairut, C., Ngowyutagon, P., Tunganuntarat, A., Khowawisetsut, L., Kittisares, K., & Prabhasawat, P. (2022). Comparison of epitheliotrophic factors in platelet-rich plasma versus autologous serum and their treatment efficacy in dry eye disease. Scientific reports, 12(1), 8906. https://doi.org/10.1038/s41598-022-12879-x
15. Poldee, S.,Metheetrairut, C., Suthammarak, W., & Trakarnsanga, K.(2024).Angiotensin II promotes erythroid proliferation in a three-stage erythroid culture system. MethodsX, 12, Article 102714. https://doi.org/10.1016/j.mex.2024.102714

1.

Metheetrairut C, Chotigavanich C, Amornpichetkul K, Keskool P, Ongard S, Thianboonsong A, Metheetrairut C. Expression Levels of miR-34-family microRNAs are Associated with TP53 Mutation Status in Head and Neck Squamous Cell Carcinoma. Meeting Abstract, October 3-6, 2018; EMBO|EMBL Symposia: The Complex Life of RNA, EMBL, Heidelberg, Germany.

2.

Metheetrairut C., Adams, B.D., and Slack, F.J. cel-mir-237 and its homologue, hsa-miR-125b, sensitize cells to ionizing radiation. Cold Spring Harbor Laboratory Meeting on Regulatory and Non-Coding RNAs: August 26th-30th, 2014, Cold Spring Harbor, NY, USA

3.

Metheetrairut C. and Slack, F.J. ACE-like non-peptidase (acn-1) as a new suppressor of let-7. 18th International C. elegans Meeting: June 22nd-26th, 2011, University of California, Los Angeles, CA, USA