Assoc. Prof. Kongtana Trakarnsanga, MD,PhD

  • M.D. (first class honor), Faculty of Medicine Siriraj Hospital, Mahidol University
  • Ph.D., School of Biochemistry, University of Bristol, UK
  • postdoctoral research assistant at School of Biochemistry, University of Bristol, UK

1021 Srisavarindira Bldg. 10th Fl. Department of Biochemistry. Faculty of Medicine, Siriraj Hospital, Bangkoknoi, Bangkok 10700

Laboratory : Srisavarindira Bldg. 9rd Fl.

Phone : (02) 419-9142

E-Mail : kongtana.tra@mahidol.ac.th

งานวิจัย

Erythropoiesis, a continuous physiological process by which mature red blood cells (RBC) are generated from multipotent stem cells, is regulated by myriad of cytokines, growth factors and extra-cellular matrix proteins via intracellular signal transduction pathways. Over the past two decades understanding of factors that regulate this process has increased, which has allowed for the development of in vitro culture systems that can on a small scale, generate RBCs from isolated stem cell populations. However, at present extrapolated cell numbers produced fall short of the level required for therapeutics, due to limited proliferation capacity. My research focuses on the identification of molecular mechanisms underlying the proliferation of erythroid cells with an aim of increasing the number of red blood cells obtained from in vitro culture system to enable development of a novel therapeutic product, as an alternative to donor blood. I am also interested to work on the generation of immortalised erythroid cell lines, and plan to use them as model cellular systems to study the molecular mechanisms of red cell disorders, eg. thalassemia. The work is in collaboration with Dr Jan Frayne at University of Bristol, Bristol, UK and Prof David Anstee at Bristol Institute for Transfusion Sciences, NHSBT, Bristol, UK.

1. Singleton, B. K., Lau, W., Fairweather, V. S., Burton, N. M., Wilson, M. C., Parsons, S. F., Richardson, B. M., Trakarnsanga, K., Brady, R. L., Anstee, D. J., & Frayne, J. (2011). Mutations in the second zinc finger of human EKLF reduce promoter affinity but give rise to benign and disease phenotypes. Blood, 118(11), 3137–3145. https://doi.org/10.1182/blood-2011-04-349985
2. Griffiths, R. E., Kupzig, S., Cogan, N., Mankelow, T. J., Betin, V. M., Trakarnsanga, K., Massey, E. J., Parsons, S. F., Anstee, D. J., & Lane, J. D. (2012). The ins and outs of human reticulocyte maturation: autophagy and the endosome/exosome pathway. Autophagy, 8(7), 1150–1151. https://doi.org/10.4161/auto.20648
3. Griffiths, R. E., Kupzig, S., Cogan, N., Mankelow, T. J., Betin, V. M., Trakarnsanga, K., Massey, E. J., Lane, J. D., Parsons, S. F., & Anstee, D. J. (2012). Maturing reticulocytes internalize plasma membrane in glycophorin A-containing vesicles that fuse with autophagosomes before exocytosis.Blood, 119(26), 6296–6306. https://doi.org/10.1182/blood-2011-09-376475
4. Trakarnsanga, K., Wilson, M. C., Lau, W., Singleton, B. K., Parsons, S. F., Sakuntanaga, P., Kurita, R., Nakamura, Y., Anstee, D. J., & Frayne, J. (2014). Induction of adult levels of β-globin in human erythroid cells that intrinsically express embryonic or fetal globin by transduction with KLF1 and BCL11A-XL.Haematologica, 99(11), 1677–1685. https://doi.org/10.3324/haematol.2014.110155
5. Trakarnsanga, K., Wilson, M. C., Griffiths, R. E., Toye, A. M., Carpenter, L., Heesom, K. J., Parsons, S. F., Anstee, D. J., & Frayne, J. (2014). Qualitative and quantitative comparison of the proteome of erythroid cells differentiated from human iPSCs and adult erythroid cells by multiplex TMT labelling and nanoLC-MS/MS. PloS one, 9(7), e100874. https://doi.org/10.1371/journal.pone.0100874
6. Wilson, M. C., Trakarnsanga, K., Heesom, K. J., Cogan, N., Green, C., Toye, A. M., Parsons, S. F., Anstee, D. J., & Frayne, J. (2016). Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Molecular & cellular proteomics : MCP, 15(6), 1938–1946. https://doi.org/10.1074/mcp.M115.057315
7. Trakarnsanga, K., Griffiths, R. E., Wilson, M. C., Blair, A., Satchwell, T. J., Meinders, M., Cogan, N., Kupzig, S., Kurita, R., Nakamura, Y., Toye, A. M., Anstee, D. J., & Frayne, J. (2017). An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells. Nature communications, 8, 14750. https://doi.org/10.1038/ncomms14750
8. Poldee, S., Metheetrairut, C., Nugoolsuksiri, S., Frayne, J., & Trakarnsanga, K. (2018). Optimization of an erythroid culture system to reduce the cost of in vitro production of red blood cells. MethodsX, 5, 1626–1632. https://doi.org/10.1016/j.mex.2018.11.018
9. Hawksworth, J., Satchwell, T. J., Meinders, M., Daniels, D. E., Regan, F., Thornton, N. M., Wilson, M. C., Dobbe, J. G., Streekstra, G. J., Trakarnsanga, K., Heesom, K. J., Anstee, D. J., Frayne, J., & Toye, A. M. (2018). Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing. EMBO molecular medicine, 10(6), e8454. https://doi.org/10.15252/emmm.201708454
10. Wattanapanitch, M., Damkham, N., Potirat, P., Trakarnsanga, K., Janan, M., U-Pratya, Y., Kheolamai, P., Klincumhom, N., & Issaragrisil, S. (2018). One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system. Stem cell research & therapy, 9(1), 46. https://doi.org/10.1186/s13287-018-0779-3
11. Trakarnsanga, K., Wilson, M. C., Heesom, K. J., Andrienko, T. N., Srisawat, C., & Frayne, J. (2018). Secretory factors from OP9 stromal cells delay differentiation and increase the expansion potential of adult erythroid cells in vitro. Scientific reports, 8(1), 1983. https://doi.org/10.1038/s41598-018-20491-1
12. Daniels, D. E., Downes, D. J., Ferrer-Vicens, I., Ferguson, D. C. J., Singleton, B. K., Wilson, M. C., Trakarnsanga, K., Kurita, R., Nakamura, Y., Anstee, D. J., & Frayne, J. (2020). Comparing the two leading erythroid lines BEL-A and HUDEP-2. Haematologica, 105(8), e389–e394. https://doi.org/10.3324/haematol.2019.229211
13. Trakarnsanga, K., Ferguson, D., Daniels, D. E., Griffiths, R. E., Wilson, M. C., Mordue, K. E., Gartner, A., Andrienko, T. N., Calvert, A., Condie, A., McCahill, A., Mountford, J. C., Toye, A. M., Anstee, D. J., & Frayne, J. (2019). Vimentin expression is retained in erythroid cells differentiated from human iPSC and ESC and indicates dysregulation in these cells early in differentiation. Stem cell research & therapy, 10(1), 130. https://doi.org/10.1186/s13287-019-1231-z
14. Netsrithong, R., Suwanpitak, S., Boonkaew, B., Trakarnsanga, K., Chang, L. J., Tipgomut, C., Vatanashevanopakorn, C., Pattanapanyasat, K., & Wattanapanitch, M. (2020). Multilineage differentiation potential of hematoendothelial progenitors derived from human induced pluripotent stem cells. Stem cell research & therapy, 11(1), 481. https://doi.org/10.1186/s13287-020-01997-w
15. Trakarnsanga, K., Tipgomut, C., Metheetrairut, C., Wattanapanitch, M., Khuhapinant, A., Poldee, S., Kurita, R., Nakamura, Y., Srisawat, C., & Frayne, J. (2020). Generation of an immortalised erythroid cell line from haematopoietic stem cells of a haemoglobin E/β-thalassemia patient. Scientific reports, 10(1), 16798. https://doi.org/10.1038/s41598-020-73991-4
17. Tipgomut, C., Khuhapinant, A., Wilson, M. C., Poldee, S., Heesom, K. J., Metheetrairut, C., Sripichai, O., Mitrpant, C., Frayne, J., & Trakarnsanga, K. (2021). MTAP-related increased erythroblast proliferation as a mechanism of polycythaemia vera. Scientific reports, 11(1), 22483. https://doi.org/10.1038/s41598-021-01877-0
18. Ferguson, D. C. J., Mokim, J. H., Meinders, M., Moody, E. R. R., Williams, T. A., Cooke, S., Trakarnsanga, K., Daniels, D. E., Ferrer-Vicens, I., Shoemark, D., Tipgomut, C., Macinnes, K. A., Wilson, M. C., Singleton, B. K., & Frayne, J. (2021). Characterization and evolutionary origin of novel C2H2 zinc finger protein (ZNF648) required for both erythroid and megakaryocyte differentiation in humans. Haematologica, 106(11), 2859–2873. https://doi.org/10.3324/haematol.2020.256347
19. Daniels, D. E., Ferguson, D. C. J., Griffiths, R. E., Trakarnsanga, K., Cogan, N., MacInnes, K. A., Mordue, K. E., Andrienko, T., Ferrer-Vicens, I., Ramos Jiménez, D., Lewis, P. A., Wilson, M. C., Canham, M. A., Kurita, R., Nakamura, Y., Anstee, D. J., & Frayne, J. (2021). Reproducible immortalization of erythroblasts from multiple stem cell sources provides approach for sustainable RBC therapeutics. Molecular therapy. Methods & clinical development, 22, 26–39. https://doi.org/10.1016/j.omtm.2021.06.002
20. Mungchan, P., Glab-Ampai, K., Chruewkamlow, N., Trakarnsanga, K., Srisawat, C., Nguyen, K. T., Chaicumpa, W., & Punnakitikashem, P. (2022). Targeted Nanoparticles for the Binding of Injured Vascular Endothelium after Percutaneous Coronary Intervention. Molecules (Basel, Switzerland), 27(23), 8144. https://doi.org/10.3390/molecules27238144
21. Trakarnsanga, K., Thongsin, N., Metheetrairut, C., Tipgomut, C., Poldee, S., & Wattanapanitch, M. (2022). Genetic correction of haemoglobin E in an immortalised haemoglobin E/beta-thalassaemia cell line using the CRISPR/Cas9 system. Scientific reports, 12(1), 15551. https://doi.org/10.1038/s41598-022-19934-7
22. Damkham, N., Lorthongpanich, C., Klaihmon, P., Lueangamornnara, U., Kheolamai, P., Trakarnsanga, K., & Issaragrisil, S. (2022). YAP and TAZ play a crucial role in human erythrocyte maturation and enucleation. Stem cell research & therapy, 13(1), 467. https://doi.org/10.1186/s13287-022-03166-7
23. Poldee, S., Metheetrairut, C., Suthammarak, W., & Trakarnsanga, K. (2024). Angiotensin II promotes erythroid proliferation in a three-stage erythroid culture system. MethodsX, 12, Article 102714. https://doi.org/10.1016/j.mex.2024.102714

1.

Trakarnsanga K, Wilson MC, Griffiths RE, Kurita R, Nakamura Y, Anstee DJ, Frayne J. The first human immortalised cell line generated from adult erythroid cells. 25th Regional Congress of the ISBT, London, UK. 2015.

2.

Griffiths RE, Trakarnsanga K, Kurita R, Nakamura Y, Frayne J, Anstee DJ. Enucleation in cultured erythroid cells from human peripheral blood and immortalised human erythroid progenitor cell lines. 25th Regional Congress of the ISBT, London, UK. 2015.

3.

Wilson MC, Trakarnsanga K, Heesom K, Chan DW, Frayne J. The development of methodology for proteome-wide profiling, and comparative analysis of transcription factors in erythroid cells. 15th International Symposium on Blood Substitutes and Oxygen Therapeutics (ISBS2015), Lund, Sweden

4.

Wilson MC, Trakarnsanga K, Heesom K, Parsons S, Anstee D, Frayne J. A quantitative and comparative proteome analysis between erythroid cells derived in vitro from adult and cord blood stem cells and mature red blood cells. Red cells Gordon Research Conference 2013, Andover, NH, USA by Gordon Research Conference.

5.

Trakarnsanga K, Wilson MC, Heesom K, Parsons S, Anstee D, Frayne J. Qualitative and quantitative, comparative proteome analysis of erythroid cells derived from human induced pluripotent stem cells with adult erythroid cells; utilizing multiplex Tandem Mass Tag labeling and mass spectrometry, Research and Development Annual Conference 2013, oxford, UK by NHS blood and transplant.

6.

Wilson MC, Trakarnsanga K, Heesom K, Parsons S, Anstee D, Frayne J. Proteomic study of erythropoiesis – advances in technology. Research and Development Annual Conference 2012, Oxford, UK by NHS blood and transplant.

7.

Trakarnsanga K, Wilson MC, Carpenter L, Griffiths RE, Parsons S, Anstee D, Frayne J. A proteomic approach to characterising erythropoiesis from induced pluripotent stem cells (iPSC). Research and Development Annual Conference 2012, Oxford, UK by NHS blood and transplant.

8.

Chantakarn S, Lohsiriwat S, Trakarnsanga K, Sunlakaviset P, Attatippaholkun N, Thanamitsomboon N, et al. Nutritional value that 2nd-6th year Siriraj medical students, academic year 2008, gain. Joint Conference in Medical Sciences. 2009:101.