Professor Patcharee Lertrit Wichyanuwat

  • M.D., Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand
  • Ph.D. (Biochemistry), Monash University, Melbourne, Australia
  • Certificated Thai Board of Family Medicine , Faculty of Medicine Siriraj Hospital

Office : 1018 Srisavarindira Bldg. 10th Fl. Department of Biochemistry. Faculty of Medicine Siriraj Hospital, Bangkoknoi, Bangkok 10700

Laboratory : Metabolic and mitochondrial laboratory, 926 Srisavarindira Bldg. 9rd Fl.

Phone : 02)-419-8735 (office), (02)-419-5361 (lab)
Fax: (02)-276-8548, (02)-419-9141

E-Mail : patcharee.ler@mahidol.ac.th, lertrito@yahoo.com

Research Profile
Glucose uptake assay in the cells derived from Diabetes Mellitus type 2 patients containing mitochondrial DNA mutation.
Background and scope

This project aims to investigate the ability of the cell (either fibroblast or transformed lymphocyte) derived from DM type II patients who carry mitochondrial mutation suspected to be the caused of this disease. The project will included either the culture of skin fibroblast or transformation of lymphocyte from the patients investigated from previous project to carry the mitochondrial mutation. Glucose uptake assay will be performed in the cell of the patients as well as normal control. The data will be analysed.

Mitochondrial DNA mutation in sensori-neural hearing loss in Thailand.
Background and scope

This project attempts to identify the mitochondrial DNA mutation in Thai population who has hearing problem. The patients who have hearing loss, sensori neural type, and positive family history will be from Department of Oto-Rhino-Laryngology, Siriraj Hospital. Total cell DNA will be extracted from whole blood samples. DNA sequencing will be performed in the rRNA coding region of mitochondrial genome. The data will be analysed compare to normal health controls.

Genetic relationship among populations speaking Tai Kadai language.
Background and scope

This project will emphasize in the population genetics of people living in Southern China and Southeast Asia. The hair follicle will be collected from various populations who speak Tai-Kadai language family in these areas and the mitochondrial genome (HVS-1 region) will be studied. The nucleotide diversity, phylogenetic tree and population tree will be constructed from their mitochondrial HVS-1 sequences. The genetic distance of inter and intra population of these people will be analysed and interpreted.

Ancient DNA analysis in 2 prehistoric populations of Thailand.
Background and scope

The purpose of this project is to investigate the genetic relationship between 2 ancient populations from Ban Non Wat, Pimai, Nakorn Ratchasima in the northeastern and from Kok Phanomdi, Chonburi, and the present population now living in Thailand. The molar tooth from the skeletal remains excavated from those sites will be used for the DNA extraction. The mitochondrial genome (HVS-1 region) will be sequenced from each sample. The nucleotide diversity, phylogenetic tree and population tree will be constructed from their mitochondrial HVS-1 sequences. The genetic distance of inter and intra population of these people together with the present populations around these areas will be performed.

1. Thyagarajan, D., Byrne, E., Noer, S., Lertrit, P., Utthanophol, P., Kapsa, R., & Marzuki, S. (1991). Mitochondrial DNA sequence analysis in congenital myotonic dystrophy. Annals of neurology, 30(5), 724–727. https://doi.org/10.1002/ana.410300514
2. Noer, A. S., Sudoyo, H., Lertrit, P., Thyagarajan, D., Utthanaphol, P., Kapsa, R., Byrne, E., & Marzuki, S. (1991). A tRNA(Lys) mutation in the mtDNA is the causal genetic lesion underlying myoclonic epilepsy and ragged-red fiber (MERRF) syndrome. American journal of human genetics, 49(4), 715–722.
3. Marzuki, S., Noer, A. S.,Lertrit, P., Thyagarajan, D., Kapsa, R., & Byrne, E. (1992). Molecular pathology of mitochondrial respiratory disorders: Normal nucleotide variants of human mitochondrial genome and mtDNA lesion in MERRF encephalomyopathy. In T. Sato (Ed.), Mitochondrial encephalomyopathies (Progress in Neuropathology, Vol. 9, pp. 131–150). New York: Raven Press.
4. Marzuki, S., Noer, A. S., Lertrit, P.,Thyagarajan, D., Kapsa, R., Utthanaphol, P., & Byrne, E. (1991). Normal variants of human mitochondrial DNA and translation products: the building of a reference data base. Human genetics, 88(2), 139–145. https://doi.org/10.1007/BF00206061
5. Marzuki, S.,Lertrit, P., Noer, A. S., Kapsa, R. M. I., Sudoyo, H., Byrne, E., & Thyagarajan, D. (1992). Reply to Howell et al.: The need for a joint effort in the construction of a reference data base for normal sequence variants of human mtDNA. American Journal of Human Genetics, 50(6), 1337–1340.
6. Lertrit, P., Noer, A. S., Jean-Francois, M. J., Kapsa, R., Dennett, X., Thyagarajan, D., Lethlean, K., Byrne, E., & Marzuki, S. (1992). A new disease-related mutation for mitochondrial encephalopathy lactic acidosis and strokelike episodes (MELAS) syndrome affects the ND4 subunit of the respiratory complex I. American journal of human genetics, 51(3), 457–468.
7. Lertrit, P., Noer, A. S., Byrne, E., & Marzuki, S. (1992). Tissue segregation of a heteroplasmic mtDNA mutation in MERRF (myoclonic epilepsy with ragged red fibers) encephalomyopathy. Human genetics, 90(3), 251–254. https://doi.org/10.1007/BF00220072
8. Howell, N., McCullough, D. A., Kubacka, I., Halvorson, S., Mackey, D., Marzuki, S., Lertrit, P., Noer, A. S., Kapsa, R. M. I., Sudoyo, H., Byrne, E., & Thyagarajan, D. (1992). The sequence of human mtDNA: The question of errors versus polymorphisms. American Journal of Human Genetics, 50(6), 1333–1340.
9. Thyagarajan, D., Byrne, E., Noer, S., Lertrit, P., Utthanophol, P., Kapsa, R., & Marzuki, S. (1993). Significance of mitochondrial DNA deletions in myotonic dystrophy. Acta neurologica Scandinavica, 87(1), 32–36. https://doi.org/10.1111/j.1600-0404.1993.tb04071.x
10. Thyagarajan, D., Byrne, E., Noer, A. S., Lertrit, P., Kapsa, R., & Marzuki, S. (1993). mtDNA in congenital myotonic dystrophy. American journal of human genetics, 52(1), 207–210.
11. Lertrit, P., Kapsa, R. M., Jean-Francois, M. J., Thyagarajan, D., Noer, A. S., Marzuki, S., & Byrne, E. (1994). Mitochondrial DNA polymorphism in disease: a possible contributor to respiratory dysfunction. Human molecular genetics, 3(11), 1973–1981. https://doi.org/10.1093/hmg/3.11.1973
12. Kotsimbos, N., Jean-Francois, M. J., Huizing, M., Kapsa, R. M., Lertrit, P., Siregar, N. C., Marzuki, S., Sue, C., & Byrne, E. (1994). Rapid and noninvasive screening of patients with mitochondrial myopathy. Human mutation, 4(2), 132–135. https://doi.org/10.1002/humu.1380040207
13. Jean-Francois, M. J.,Lertrit, P., Berkovic, S. F., Crimmins, D., Morris, J., Marzuki, S., & Byrne, E. (1994). Heterogeneity in the phenotypic expression of the mutation in the mitochondrial tRNA(Leu) (UUR) gene generally associated with the MELAS subset of mitochondrial encephalomyopathies. Australian and New Zealand journal of medicine, 24(2), 188–193. https://doi.org/10.1111/j.1445-5994.1994.tb00556.x
14. Atchaneeyasakul, L., Lertrit, P., Devahastin, V., & Sangruchi, T. (1994). Case report: Kearns–Sayre syndrome. Thai Journal of Ophthalmology, 8, 145–153.
15. Marzuki, S., Sudoyo, H., & Lertrit, P. (1995). Update in molecular genetics: mitochondrial energy transduction disorders. The Southeast Asian journal of tropical medicine and public health, 26 Suppl 1, 155–161.
16. Lertrit, P., Atchaneeyasakul, L., Devahastin, V., Saechan, V., Sangruchi, T., Neungton, N., & Lekhakula, S. (1995). Mitochondrial genome analysis in Kearns-Sayre syndrome. The Southeast Asian journal of tropical medicine and public health, 26 Suppl 1, 162–165.
17. Lekhakula, S., Lertrit, P., Tuchinda, C., Angsusingha, K., Kangsadalampai, S., Wacharasindhu, S., Futrakul, A., & Sritawil, K. (1995). Molecular examination of GH gene deletion in familial growth hormone deficiency. The Southeast Asian journal of tropical medicine and public health, 26 Suppl 1, 207–211.
18. Kapsa, R. M., Jean-Francois, M. J., Lertrit, P., Weng, S., Siregar, N., Ojaimi, J., Donnan, G., Masters, C., & Byrne, E. (1996). Mitochondrial DNA polymorphism in substantia nigra.Journal of the neurological sciences, 144(1-2), 204–211. https://doi.org/10.1016/s0022-510x(96)00247-x
19. Siripurkpong, P., Harnyuttanakorn, P., Chindaduangratana, C., Kotchabhakdi, N.,Wichyanuwat, P., & Casalotti, S. O. (1997). Dexamethasone, but not stress, induce measurable changes of mitochondrial benzodiazepine receptor mRNA levels in rats. European journal of pharmacology, 331(2-3), 227–235. https://doi.org/10.1016/s0014-2999(97)01039-x
20. Lertrit, P., Imsumran, A., Trongpanich, Y., Karnkirawattana, P., Devahasdin, V., Atchaneeyasakul, L., & others. (1998). Mitochondrial genetics of mitochondrial diseases in Thailand. Siriraj Hospital Gazette, 50, 53–64.
21. Dumrongdej, P., Lertrit, P., Wongmanee, U., Panichkul, N., & Neungton, N. (1998). Serum ceruloplasmin in Wilson's disease. Siriraj Hospital Gazette, 50, 576–581.
22. Lertrit, P., Ruangvaravate, N., Trongpanich, Y., Imsumran, A., Mungkornkarn, C., & Neungton, N. (1999). Leber's hereditary optic neuropathy (LHON) with mitochondrial ND4 gene mutation (11778) in a Thai patient. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 82(1), 59–64.
23. Lertrit, P., Imsumran, A., Karnkirawattana, P., Devahasdin, V., Sangruchi, T., Atchaneeyasakul, L., Mungkornkarn, C., & Neungton, N. (1999). A unique 3.5-kb deletion of the mitochondrial genome in Thai patients with Kearns-Sayre syndrome. Human genetics, 105(1-2), 127–131. https://doi.org/10.1007/s004399900062
24. Lertrit, P., Choopayak, C., Udomsangpetch, R., Chansiri, K., & Mungkornkarn, C. (1999). The mitochondrial cytochrome oxidase subunit III gene and protein sequence analysis in chloroquine-resistant Plasmodium falciparum. Journal of Biochemistry, Molecular Biology and Biophysics, 3(3-4), 161-170.
25. Sitburana, O., Witoonpanich, R., Phudhichareonrat, S., Lertrit, P., & Supavilai, R. (2001). Seizures in myoclonic epilepsy with ragged-red fibers detected by DNA analysis: a case report. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 84(7), 1051–1055.
26. Senanarong, V., Harnphadungkit, K., Lertrit, P., Mitrpant, C., Udompunthurak, S., Limwong, C., Prayoonwiwat, N., & Poungvarin, N. (2001). Experience of ApoE study in Thai elderly. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 84(2), 182–187.
27. Chuenkongkaew, W. L., Lertrit, P., Poonyathalang, A., Sura, T., Ruangvaravate, N., Atchaneeyasakul, L., & Suphavilai, R. (2001). Leber's hereditary optic neuropathy in Thailand. Japanese journal of ophthalmology, 45(6), 665–668. https://doi.org/10.1016/s0021-5155(01)00423-3
28. Sudoyo, H., Suryadi, H., Lertrit, P., Pramoonjago, P., Lyrawati, D., & Marzuki, S. (2002). Asian-specific mtDNA backgrounds associated with the primary G11778A mutation of Leber's hereditary optic neuropathy. Journal of human genetics, 47(11), 594–604. https://doi.org/10.1007/s100380200091
29. Ueyama, H., Li, Y. H., Fu, G. L., Lertrit, P., Atchaneeyasakul, L. O., Oda, S., Tanabe, S., Nishida, Y., Yamade, S., & Ohkubo, I. (2003). An A-71C substitution in a green gene at the second position in the red/green visual-pigment gene array is associated with deutan color-vision deficiency.Proceedings of the National Academy of Sciences of the United States of America, 100(6), 3357–3362. https://doi.org/10.1073/pnas.0637437100
30. Witoonpanich, R., Phankhian, S., Sura, T.,Lertrit, P., & Phudhichareonrat, S. (2004). Oculopharyngodistal myopathy in a Thai family. Journal of the Medical Association of Thailand = Chotmaihet thangphaet, 87(12), 1518–1521.
31. Chuenkongkaew, W., Lertrit, P., & Suphavilai, R. (2004). Case report: A Thai patient with Leber's hereditary optic neuropathy linked to mitochondrial DNA 14484 mutation. The Southeast Asian journal of tropical medicine and public health, 35(1), 167–168.
32. Chuenkongkaew, W. L., Suphavilai, R., Vaeusorn, L., Phasukkijwatana, N., Lertrit, P., & Suktitipat, B. (2005). Proportion of 11778 mutant mitochondrial DNA and clinical expression in a thai population with leber hereditary optic neuropathy. Journal of neuro-ophthalmology : the official journal of the North American Neuro-Ophthalmology Society, 25(3), 173–175. https://doi.org/10.1097/01.wno.0000176631.87234.49
33. Chuenkongkaew, W. L., Samsen, P.,Lertrit, P.,& Thanasombatsakul, N. (2005). An unusual electrophysiologic finding in acute stage of Leber’s hereditary optic neuropathy. Neuro-Ophthalmology Japan, 22(3), 454–458.
34. Chuenkongkaew, W. L., Lertrit, P., Limwongse, C., Nilanont, Y., Boonyapisit, K., Sangruchi, T., & et al. (2005). An unusual family with Leber's hereditary optic neuropathy and facioscapulohumeral muscular dystrophy. European Journal of Neurology, 12(5), 388–391. https://doi.org/10.1111/j.1468-1331.2005.01003.x
35. Tharaphan, P., Chuenkongkaew, W. L., Luangtrakool, K., Sanpachudayan, T., Suktitipat, B., Suphavilai, R., Srisawat, C., Sura, T., & Lertrit, P. (2006). Mitochondrial DNA haplogroup distribution in pedigrees of Southeast Asian G11778A Leber hereditary optic neuropathy. Journal of neuro-ophthalmology : the official journal of the North American Neuro-Ophthalmology Society, 26(4), 264–267. https://doi.org/10.1097/01.wno.0000249318.88991.c4
36. Phasukkijwatana, N., Chuenkongkaew, W. L., Suphavilai, R., Suktitipat, B., Pingsuthiwong, S., Ruangvaravate, N., Atchaneeyasakul, L. O., Warrasak, S., Poonyathalang, A., Sura, T., & Lertrit, P.(2006). The unique characteristics of Thai Leber hereditary optic neuropathy: analysis of 30 G11778A pedigrees. Journal of human genetics, 51(4), 298–304. https://doi.org/10.1007/s10038-006-0361-1
37. Phasukkijwatana, N., Chuenkongkaew, W. L., Suphavilai, R., Luangtrakool, K., Kunhapan, B., & Lertrit, P. (2006). Transmission of heteroplasmic G11778A in extensive pedigrees of Thai Leber hereditary optic neuropathy. Journal of human genetics, 51(12), 1110–1117. https://doi.org/10.1007/s10038-006-0073-6
38. Luangtrakool, K., Tate, F. Y., Shepherd, R., Campbell, S., Sue, C. M., &Lertrit, P. (2008). Characterization of 2-deoxy-D-glucose uptake in fibroblast cultures derived from patients with A3243G mitochondrial DNA mutation. The Southeast Asian journal of tropical medicine and public health, 39(4), 745–752.
39. Lertrit, P., Poolsuwan, S., Thosarat, R., Sanpachudayan, T., Boonyarit, H., Chinpaisal, C., & Suktitipat, B.(2008). Genetic history of Southeast Asian populations as revealed by ancient and modern human mitochondrial DNA analysis.American journal of physical anthropology, 137(4), 425–440. https://doi.org/10.1002/ajpa.20884
40. Phasukkijwatana, N., Kunhapan, B., Stankovich, J., Chuenkongkaew, W. L., Thomson, R., Thornton, T., Bahlo, M., Mushiroda, T., Nakamura, Y., Mahasirimongkol, S., Tun, A. W., Srisawat, C., Limwongse, C., Peerapittayamongkol, C., Sura, T., Suthammarak, W., & Lertrit, P. (2010). Genome-wide linkage scan and association study of PARL to the expression of LHON families in Thailand. Human genetics, 128(1), 39–49. https://doi.org/10.1007/s00439-010-0821-8
41. Phasukkijwatana, N., Lertrit, P., Liammongkolkul, S., & Prabhasawat, P. (2011). Stability of epitheliotrophic factors in autologous serum eye drops from chronic Stevens-Johnson syndrome dry eye compared to non-autoimmune dry eye. Current eye research, 36(9), 775–781. https://doi.org/10.3109/02713683.2011.587935
42. Kaewsutthi, S., Phasukkijwatana, N., Joyjinda, Y., Chuenkongkaew, W., Kunhapan, B., Tun, A. W., Suktitipat, B., & Lertrit, P. (2011). Mitochondrial haplogroup background may influence Southeast Asian G11778A Leber hereditary optic neuropathy. Investigative ophthalmology & visual science, 52(7), 4742–4748. https://doi.org/10.1167/iovs.10-5816
43. Pongtepaditep, S., Limjindaporn, T., Lertrit, P., Srisawat, C., & Limwongse, C. (2012). Polyglutamined expanded androgen receptor interacts with chaperonin CCT. European journal of medical genetics, 55(11), 599–604. https://doi.org/10.1016/j.ejmg.2012.06.013
44. Istikharah, R., Tun, A. W., Kaewsutthi, S., Aryal, P., Kunhapan, B., Katanyoo, W., Chuenkongkaew, W., & Lertrit, P. (2013). Identification of the variants in PARL, the nuclear modifier gene, responsible for the expression of LHON patients in Thailand. Experimental eye research, 116, 55–57. https://doi.org/10.1016/j.exer.2013.08.007
45. Tun, A. W., Chaiyarit, S., Kaewsutthi, S., Katanyoo, W., Chuenkongkaew, W., Kuwano, M., Tomonaga, T., Peerapittayamongkol, C., Thongboonkerd, V., & Lertrit, P. (2014). Profiling the mitochondrial proteome of Leber's Hereditary Optic Neuropathy (LHON) in Thailand: down-regulation of bioenergetics and mitochondrial protein quality control pathways in fibroblasts with the 11778G>A mutation. PloS one, 9(9), e106779. https://doi.org/10.1371/journal.pone.0106779
46. Ranaweera, L., Kaewsutthi, S., Tun, A. W., Boonyarit, H., Poolsuwan, S., & Lertrit, P. (2014). Mitochondrial DNA history of Sri Lankan ethnic people: Their relations within the island and with the Indian subcontinental populations. Journal of Human Genetics, 59(1), 28–36. https://doi.org/10.1038/jhg.2013.112
47. Boonyarit, H., Mahasirimongkol, S., Chavalvechakul, N., Aoki, M., Amitani, H., Hosono, N., Kamatani, N., Kubo, M., & Lertrit, P. (2014). Development of a SNP set for human identification: A set with high powers of discrimination which yields high genetic information from naturally degraded DNA samples in the Thai population. Forensic science international. Genetics, 11, 166–173. https://doi.org/10.1016/j.fsigen.2014.03.010
48. Kaewsutthi, S., Santiprabhob, J., Phonrat, B., Tungtrongchitr, A., Lertrit, P., & Tungtrongchitr, R. (2016). Exome sequencing in Thai patients with familial obesity.Genetics and molecular research : GMR, 15(2), 10.4238/gmr.15028311. https://doi.org/10.4238/gmr.15028311
49. Jinda, W., Poungvarin, N., Taylor, T. D., Suzuki, Y., Thongnoppakhun, W., Limwongse, C., Lertrit, P., Suriyaphol, P., & Atchaneeyasakul, L. O. (2016). A novel start codon mutation of the MERTK gene in a patient with retinitis pigmentosa. Molecular vision, 22, 342–351.
50. Jinda, W., Taylor, T. D., Suzuki, Y., Thongnoppakhun, W., Limwongse, C., Lertrit, P., Trinavarat, A., & Atchaneeyasakul, L. O. (2017). Whole Exome Sequencing in Eight Thai Patients With Leber Congenital Amaurosis Reveals Mutations in the CTNNA1 and CYP4V2 Genes. Investigative ophthalmology & visual science, 58(4), 2413–2420. https://doi.org/10.1167/iovs.16-21322
51. McColl, H., Racimo, F., Vinner, L., Demeter, F., Gakuhari, T., Moreno-Mayar, J. V., van Driem, G., Gram Wilken, U., Seguin-Orlando, A., de la Fuente Castro, C., Wasef, S., Shoocongdej, R., Souksavatdy, V., Sayavongkhamdy, T., Saidin, M. M., Allentoft, M. E., Sato, T., Malaspinas, A. S., Aghakhanian, F. A., Korneliussen, T., … Willerslev, E. (2018). The prehistoric peopling of Southeast Asia. Science (New York, N.Y.), 361(6397), 88–92. https://doi.org/10.1126/science.aat3628
52. Veenin, K., Wattanasirichaigoon, D., Suktitipat, B., Noojarern, S., Lertrit, P., Tim-Aroon, T., Kaewsutthi, S., & Treepongkaruna, S. (2022). Association of Mitochondrial DNA Polymorphisms With Pediatric-Onset Cyclic Vomiting Syndrome. Frontiers in pediatrics, 10, 876436. https://doi.org/10.3389/fped.2022.876436

1.

Witoonpanich R, Jindahra P, Lertrit P, et al. Autosomal dominant progressive external ophthalmoplegia: Report of a Thai family. NEUROMUSCULAR DISORDERS 2008;18:753.