Laboratory Techniques in Molecular Biology (SIBB 510)credit 2(0-4-2)

Laboratory Techniques in Medical Biochemistry (SIBB 511)credit 1(0-2-1)

Tuesday, Thursday, and Friday, 1.00 – 4.00 pm.
Biochemistry Conference Room2, 10th Fl. Srisavarindira building
Course coordinator: Dr.Prinmana Punnakitikashem
Phone 02-4199133 E-mail: primana.pun@mahidol.ac.th

Course description SIBB510

The techniques used in molecular biology, DNA extraction, DNA electrophoresis, DNA technology, application of polymerase chain reaction in biochemistry of gene expression, cytogenetic techniques, monoclonal antibody application, flow cytometry, application of bioinformatics

Course description SIBB511

The techniques used in medical biochemistry, buffer preparation, spectrophotometry, spectrofluorometry, gel-filtration chromatography, high performance liquid chromatography, mass spectrometry, tissue culture techniques, tissue homogenization, protein isolation and purification, protein electrophoresis, analysis of enzyme in electron transport system

Major Expected Learning Outcomes

1.1 Students should have moral, professional, and research ethics.
1.2 Students should be disciplined, punctual, and responsible and should follow university rules.
1.3 Students should respect the rights and opinions of others. (M.Sc.)
2.1 Students should have profound knowledge of Biochemistry and Medical Molecular Biology
2.2 Students should be able to study independently.
2.3 Students should possess intellectual curiosity and be able to keep him/herself up to date.
3.1 Students should be able to analyze theoretical data from classes and from other sources.
3.2 Students should be able to analyze and interpret research data.
4.1 Students should be able to work with others and have a good working relationship.
5.1 Students should be able to use information technology and computers to search for relevant information, have a good judgment when analyzing any information, and present it appropriately.
5.2 Students should be able to use English for communication and presentation of academic work.
5.3 Students should have an adequate knowledge in statistical analysis for academic and research work.
6.1 Students should perform any laboratory work in Biochemistry and Medical Molecular Biology appropriately, decently, and efficiently.
2.5 Students should be able to apply the knowledge for real-life situation.(Ph.D.)

Course Schedule:

Academic year 2025
New Update on 29 July 2025

Course Materials

PCR
Lab handout
(Download)

Real-time PCR
Lab handout
(Download)