Assoc. Prof. Vorapan Sirivatanauksorn, MD, PhD

  • M.D., Faculty of Medicine Siriraj Hospital, Mahidol University
  • Ph.D. (Biochemistry), Imperial College School of Medicine, University of London, UK.

Office : 1019 Srisavarindira Bldg. 10th Fl. Department of Biochemistry. Faculty of Medicine Siriraj Hospital, Bangkoknoi, Bangkok 10700

Laboratory : 917 Srisavarindira Bldg. 9rd Fl.

Phone : (02) 419-9140

E-Mail : vorapan.sir@mahidol.ac.th

Research Profile

HepaCAM is a new Ig-like adhesion molecule. This novel protein displays the typical structure of the adhesion molecules in immunoglobulin superfamily (IgSF). Revealed by cell spreading and motility assays, hepaCAM is capable of modulating cell–matrix interactions, supporting hepaCAM to be an adhesion molecule. The frequent loss of hepaCAM expression in HCC together with the antiproliferative effect of hepaCAM meets the most important criteria widely used to define tumor suppressor. In addition, hepaCAM is mapped to the human chromosome 11q24. Molecular genetic and cytogenetic studies have indicated that the long arm of chromosome 11 is one of the most common targets for chromosomal aberrations during the progression of human malignancies. We speculate that loss of hepaCAM expression might play a role in the development or progression of cholangiocarcinoma as well.

Thus, we aim to evaluate cholangiocarcinoma cell lines and tissue samples of cholangiocarcinoma for loss of hepaCAM expression by both immunohistochemical and reverse transcription-polymerase chain reaction (RT-PCR) analysis.

1. Sirivatanauksorn, V., & Lemoine, N. R. (1998). Polymorphic genomic fingerprinting of synchronous hepatocellular cancers identified by arbitrarily primed-polymerase chain reaction (AP-PCR). Journal of Hepatology, 28(Suppl 1), 88.
2. Sirivatanauksorn, Y., Sirivatanauksorn, V., Bhattacharya, S., Lemoine, N. R., Tebbutt, S. J., Kakkar, A. K., et al. (1998). Clonal evolution in hepatocellular carcinomas. British Journal of Surgery, 85(11), 1577.
3. Sirivatanauksorn, Y.,Sirivatanauksorn, V. Bhattacharya, S., Davidson, B. R., Lemoine, N. R., Williamson, R. C. N., et al. (1998). Clonal evolution in hepatocellular carcinomas: Relationship to tumour size. Hepato-Gastroenterology, 45(Suppl II), LXXXIII.
4. Sirivatanauksorn, Y., Bhattacharya, S., Sirivatanauksorn, V., Lemoine, N. R., Savage, K., Dhillon, A. P., et al. (1998). Genomic heterogeneity in primary and metastatic hepatocellular carcinoma. Hepato-Gastroenterology, 45(Suppl II), CXVIII.
5. Sirivatanauksorn, V., Sirivatanauksorn, Y., & Lemoine, N. R. (1998). Molecular pattern of ductal pancreatic cancer. Langenbeck's archives of surgery, 383(2), 105–115. https://doi.org/10.1007/s004230050101
6. Sirivatanauksorn, V., McKie, A. B., & Lemoine, N. R. (1998). DNA fingerprinting strategies to identify novel genetic events in pancreatic cancer. Gastroenterology, 114(4 Suppl), A681.
7. Sirivatanauksorn, Y., Sirivatanauksorn, V., Bhattacharya, S., Davidson, B. R., Dhillon, A. P., Kakkar, A. K., Williamson, R. C., & Lemoine, N. R. (1999). Genomic heterogeneity in synchronous hepatocellular carcinomas. Gut, 45(5), 761–765. https://doi.org/10.1136/gut.45.5.761
8. Sirivatanauksorn, Y.,Sirivatanauksorn, V.,, Bhattacharya, S., Davidson, B. R., Dhillon, A. P., Kakkar, A. K., Williamson, R. C., & Lemoine, N. R. (1999). Evolution of genetic abnormalities in hepatocellular carcinomas demonstrated by DNA fingerprinting. The Journal of pathology, 189(3), 344–350. https://doi.org/10.1002/(SICI)1096-9896(199911)189:3<344::AID-PATH430>3.0.CO;2-G
9. Sirivatanauksorn V. Molecular Profiles of Pancreatic Cancer (editorial). Hosp Gaz 2000;52:776-80.
10. Sirivatanauksorn, Y., Sirivatanauksorn, V., Lemoine, N. R., Williamson, R. C., & Davidson, B. R. (2001). Genomic homogeneity in fibrolamellar carcinomas. Gut, 49(1), 82–86. https://doi.org/10.1136/gut.49.1.82
11. Sirivatanauksorn, V., Sirivatanauksorn, Y., Gorman, P. A., Davidson, J. M., Sheer, D., Moore, P. S., Scarpa, A., Edwards, P. A., & Lemoine, N. R. (2001). Non-random chromosomal rearrangements in pancreatic cancer cell lines identified by spectral karyotyping. International journal of cancer, 91(3), 350–358. https://doi.org/10.1002/1097-0215(200002)9999:9999<::aid-ijc1049>3.3.co;2-3
12. Sirivatanauksorn, Y.,Sirivatanauksorn, V., & Lemoine, N. R. (2002). DNA fingerprinting from cells captured by laser microdissection. Methods in enzymology, 356, 289–294. https://doi.org/10.1016/s0076-6879(02)56942-1
13. Sirivatanauksorn, V., Sirivatanauksorn, Y., McKie, A.B., Lemoine, N.R.(2002). Use of DNA Fingerprinting to Detect Genetic Rearrangements in Human Cancer. Methods in Molecular Medicine, vol 68. Humana Press. https://doi.org/10.1385/1-59259-135-3:107
14. Sirivatanauksorn, V. , Sirivatanauksorn, Y. , & Sattawatthamrong, Y. (2002). Pancreatic Cancer : From Clinical to Molecular Aspects. Siriraj Medical Journal, 54(10), 638–654. retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/245321
15. Sirivatanauksorn, V., Trachu, N. , Sirivatanuksorn, Y., Ratanawichitrasin, A., & Bhothisuwan, K. (2004). Molecular Detection of the Mammaglobin Expression in the Peripheral Blood of Thai Breast Cancer Patients. Siriraj Medical Journal, 56(10-12), 561–569. retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/245570
16. Choavaratana, R., Mingmitpattanakul, P., Sirivatanauksorn, V., Makemaharn, O., Petyin, S. (2008).Correlation between hyaluronan binding assay and TUNEL assay for sperm DNA integrity identification. Siriraj Med J 2008;60:334-8.
17. Chuthapisith, S., Permsapaya, W., Warnnissorn, M., Akewanlop, C., Sirivatanauksorn, V., & Prasarttong Osoth, P. (2012). Breast cancer subtypes identified by the ER, PR and HER-2 status in Thai women. Asian Pacific journal of cancer prevention : APJCP, 13(2), 459–462. https://doi.org/10.7314/apjcp.2012.13.2.459
18. Ratchatawipasanan, C., Sirivatanauksorn, V., Srisawat, C., & Chuthapisith, S. (2012). Roles of circulating tumour cells in breast cancer. Journal of the Association of General Surgeons Thailand, 8(17), 44–48.
19. Sirivatanauksorn, Y., Sirivatanauksorn, V., Srisawat, C., Khongmanee, A., & Tongkham, C. (2012). Differential expression of sprouty genes in hepatocellular carcinoma. Journal of surgical oncology, 105(3), 273–276. https://doi.org/10.1002/jso.22095
20. Sirijatuphat, R., Limmahakhun, S., Sirivatanauksorn, V., Nation, R. L., Li, J., & Thamlikitkul, V. (2015). Preliminary clinical study of the effect of ascorbic acid on colistin-associated nephrotoxicity. Antimicrobial agents and chemotherapy, 59(6), 3224–3232. https://doi.org/10.1128/AAC.00280-15
21. Sirivatanauksorn, V., Dumronggittigule, W., Dulnee, B., Srisawat, C., Sirivatanauksorn, Y., Pongpaibul, A., Masaratana, P., Somboonyosdech, C., Sripinitchai, S., Kositamongkol, P., Mahawithitwong, P., Tovikkai, C., Sangserestid, P., & Limsrichamrern, S. (2020). Role of stratifin (14-3-3 sigma) in adenocarcinoma of gallbladder: A novel prognostic biomarker. Surgical oncology, 32, 57–62. https://doi.org/10.1016/j.suronc.2019.10.022
22. Pho-Iam, T., Punnakitikashem, P., Somboonyosdech, C., Sripinitchai, S., Masaratana, P., Sirivatanauksorn, V., Sirivatanauksorn, Y., Wongwan, C., Nguyen, K. T., & Srisawat, C. (2021). PLGA nanoparticles containing α-fetoprotein siRNA induce apoptosis and enhance the cytotoxic effects of doxorubicin in human liver cancer cell line. Biochemical and biophysical research communications, 553, 191–197. https://doi.org/10.1016/j.bbrc.2021.03.086
23. Hatairaktham, S., Masaratana, P., Hantaweepant, C., Srisawat, C., Sirivatanauksorn, V., Siritanaratkul, N., Panichkul, N., & Kalpravidh, R. W. (2021). Curcuminoids supplementation ameliorates iron overload, oxidative stress, hypercoagulability, and inflammation in non-transfusion-dependent β-thalassemia/Hb E patients. Annals of hematology, 100(4), 891–901. https://doi.org/10.1007/s00277-020-04379-7
24. Punuch, K., Wongwan, C., Jantana, S., Somboonyosdech, C., Rodponthukwaji, K., Kunwong, N., Nguyen, K. T., Sirivatanauksorn, V., Sirivatanauksorn, Y., Srisawat, C., & Punnakitikashem, P. (2022). Study of siRNA Delivery via Polymeric Nanoparticles in Combination with Angiogenesis Inhibitor for The Treatment of AFP-Related Liver Cancer. International journal of molecular sciences, 23(20), 12666. https://doi.org/10.3390/ijms232012666
25. Manokasemsan, W., Jariyasopit, N., Poungsombat, P., Kaewnarin, K., Wanichthanarak, K., Kurilung, A., Duangkumpha, K., Limjiasahapong, S., Pomyen, Y., Chaiteerakij, R., Tansawat, R., Srisawat, C., Sirivatanauksorn, Y., Sirivatanauksorn, V., & Khoomrung, S. (2024).Quantifying fecal and plasma short-chain fatty acids in healthy Thai individuals. Computational and structural biotechnology journal, 23, 2163–2172. https://doi.org/10.1016/j.csbj.2024.05.007

1.

Summathed T, Manaso J, Sirivatanauksorn V, Srisawat C. Identification of the RNA motif capable of interacting with nickel. งานประชุม วิชาการประจำปี 2553 “โรงเรียนแพทย์ศิริราชเพื่อสังคม 120 ปีแห่งความก้าวหน้า” ระหว่างวันที่ 16 – 20 สิงหาคม 2553 ณ คณะแพทยศาสตร์ศิริราชพยาบาล มหาวิทยาลัยมหิดล

2.

Khongmanee A, Sirivatanauksorn Y, Srisawat C, Tongkham C, Sirivatanauksorn V. Expression of sprouty genes in hepatocellular carcinoma. Joint Conference in Medical Sciences. 2009:32.