Category Archives: Research Innovation

Factors associated with thiamin deficiency in pediatric patients with heart disease and receiving diuretics: a single-center study

Phakwan Laohathai, Rathaporn Sumboonnanonda, Puthita Saengpanit, Chodchanok Vijarnsorn, Chatchawan Srisawat , Kwanjai Chotipanang, Sarawut Junnu , Supawan Kunnangja, Hathaichanok Rukprayoon, Phakkanan Phuangphan, Sompong Liammongkolkul , Arthima Phaokong, Narumon Densupsoontorn Abstract Background: Thiamin deficiency (TD) manifesting clinically as wet beriberi can significantly impair a patient’s cardiac function. Children with heart disease who are receiving diuretic treatment […]

PlyCYU endolysin targeting Streptococcus agalactiae exhibits a CHAP activity and a glucosaminidase domain mediating multimerization

Sakunrat Ubonprasert, Wachiraporn Wachiradusit, Wichai Pornthanakasem, Warangkhana Songsungthong, Aritsara Jaruwat, Sasina Premjaichon, Tanaporn Uengwetwanit, Rinrada Suntivich, Konrawee Thananon, Kanyarat Suksomjaisaman, Jeerus Sucharitakul, Chutathip Puyprom, Tamonwan Lotangchanintra, Kanokwan Salamteh, Kittikhun Wangkanont, Channarong Rodkhum, Wonnop Visessanguan , Pimchai Chaiyen, Penchit Chitnumsub, Ubolsree Leartsakulpanich Abstract Bacteriophage endolysins are attractive alternatives to antibiotics owing to their rapid action, host […]

MicroRNA profiles of four induced pluripotent stem cell lines derived from distinct tissues

Kongtana Trakarnsanga , Nutchavadee Vorasan, Nutnicha Tantarungsee , Methichit Wattanapanitch, Chinnavuth Vatanashevanopakorn, Bhoom Suktitipat , Chanatip Metheetrairut Abstract Objective: Induced pluripotent stem cells (iPSCs) are generated from a vast number of adult cell types. While they all acquired embryonic stem cell (ESC)-like properties during reprogramming, differences in certain characteristics, including differentiation potential, remained. These differences […]

LC-QTOF-MSE with MS1-based precursor ion quantification and SiMD-assisted identification enhances human urine metabolite analysis

Alongkorn Kurilung, Suphitcha Limjiasahapong, Kwanjeera Wanichthanarak, Weerawan Manokasemsan, Khwanta Kaewnarin, Kassaporn Duangkumpha, Siriphan Manocheewa, Rossarin Tansawat, Roongruedee Chaiteerakij, Intawat Nookaew, Yongyut Sirivatanauksorn, Sakda Khoomrung Abstract This study presents the development and validation of a liquid chromatography-quadrupole-time-of-flight mass spectrometry method with data-independent acquisition (LC-QTOF-MSE) for targeted quantification, post-targeted screening, and untargeted metabolite profiling. Using MS1-based precursor […]

Novel nuclease-resistant RNA aptamer for polyhistidine-tagged proteins and its application in electrochemical aptasensor detection

Sirinapa Sripinitchai , Benchaporn Lertanantawong, Thanyarat Chaibun, Chamras Promptmas, Charin Thawornkuno, Uraiwan Kositanont, Chatchawan Srisawat Abstract The polyhistidine (His) tag is one of the most widely used tags for protein expression, detection, and purification. Aptamers targeting the His tag have been developed for various applications, including western blotting, flow cytometry, immunohistochemistry, and immunoprecipitation, due to […]

Metformin modulates the unfolded protein responses, altering lifespan and health-promoting effects in UPR-activated worms

Jerald Tan, Chutipong Chiamkunakorn, Kanpapat Boonchuay, Yiying Shi, Bart P Braeckman, Wichit Suthammarak Abstract Metformin has been demonstrated to extend lifespan in various model organisms, and its molecular effects are observed in the cytoplasm and multiple organelles, including mitochondria. However, its association with the unfolded protein response (UPR) and its impact on stress resistance and […]

Thiamin deficiency in children with chronic kidney disease on peritoneal dialysis and its association with dialysis duration and transport peritoneal membrane status

Wipawee Suwanboriboon, Thanaporn Chaiyapuk, Intraparch Tinnabut, Gornmigar Sanpawitayakul, Chatchawan Srisawat , Sarawut Junnu, Sompong Liammongkolkul, Kwanjai Chotipanang, Hathaichanok Rukprayoon, Phakwan Laohathai, Narumon Densupsoontorn Abstract Background: Patients with chronic kidney disease (CKD) stage 5D receiving peritoneal dialysis (PD) are at risk for thiamin deficiency (TD). This study compared the proportion of TD in pediatric CKD patients […]